Estrogen-responsive genes in macrophages of the bony fish gilthead seabream: a transcriptomic approach. | - CCMAR -

Journal Article

TítuloEstrogen-responsive genes in macrophages of the bony fish gilthead seabream: a transcriptomic approach.
Publication TypeJournal Article
AuthorsLiarte, S, Chaves-Pozo, E, Abellán, E, Meseguer, J, Mulero, V, Canario, AVM, García-Ayala, A
Year of Publication2011
JournalDev Comp Immunol
Volume35
Questão8
Date Published2011 Aug
Pagination840-9
ISSN1879-0089
Palavras-chaveActins, Animals, Cells, Cultured, Computer Simulation, Estrogens, Expressed Sequence Tags, Fish Proteins, Gene Expression, Gene Expression Profiling, Genes, MHC Class I, Intercellular Signaling Peptides and Proteins, Macrophages, Matrix Metalloproteinase 13, Receptors, Immunologic, Reverse Transcriptase Polymerase Chain Reaction, Sea Bream, Sequence Analysis, DNA, Transcription, Genetic
Abstract

The role of sex steroids in the modulation of fish immune responses has received little attention. Previous studies have demonstrated that 17β-estradiol (E(2)) is able to alter the response of gilthead seabream leukocytes to infectious agents. We have used suppression subtractive hybridization to identify genes upregulated by E(2) (50 ng/ml) in macrophage cultures from gilthead seabream. We isolated 393 up-regulated cDNA fragments that led to the identification of 162 candidate estrogen-responsive genes. Functional analyses revealed the presence of several enriched immune processes and molecular pathways. The E(2) up-regulation of some immune-relevant genes was further confirmed by real time RT-PCR. Bioinformatics analysis revealed the ability of E(2) to orchestrate profound alterations in the macrophage expression profile, especially immune-related processes and pathways. This is the first report on E(2)-dependent modifications of fish macrophage transcriptome and lends weight to a suggested role for estrogen in the immune system, the possible significance of which is discussed.

DOI10.1016/j.dci.2011.03.015
Sapientia

http://www.ncbi.nlm.nih.gov/pubmed/21420425?dopt=Abstract

Alternate JournalDev. Comp. Immunol.
PubMed ID21420425
CCMAR Authors