Immunochemical detection of parathyroid hormone-related protein in the saccus vasculosus of a teleost fish. | - CCMAR -

Journal Article

TítuloImmunochemical detection of parathyroid hormone-related protein in the saccus vasculosus of a teleost fish.
Publication TypeJournal Article
AuthorsDevlin, AJ, Danks, JA, Faulkner, MK, Power, DM, Canario, AVM, Martin, TJ, Ingleton, PM
Year of Publication1996
JournalGen Comp Endocrinol
Date Published1996 Jan
Palavras-chaveAnimals, Blotting, Western, Brain, Brain Chemistry, Electrophoresis, Polyacrylamide Gel, Humans, Immunohistochemistry, Parathyroid Hormone, Parathyroid Hormone-Related Protein, Perciformes, Proteins

Using antisera to regions of human parathyroid hormone-related protein (PTHrP) the saccus vasculosus (SV) of the sea bream (Sparus aurata) has been shown to contain immunoreactive PTHrP. By immunohistochemistry (IHC) the epithelial coronet cells in fixed and wax-embedded SV tissue reacted with antisera to the prepro region of human PTHrP (-13 to +2), the N-terminus PTHrP (1-16), and the midmolecule PTHrP (50-69). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of saccus extracts and incubation media contained two major proteins of 14.3 and 15 kDa. By Western blotting these two proteins both reacted with the three antisera used for IHC, suggesting that they are immunochemically similar to human PTHrP (1-84). Ultrastructurally the coronet cells of Sparus saccus vasculosus resembled coronet cells described for other teleosts, with an abundant smooth endoplasmic reticulum (SER) which was more highly organized in the coronets. IHC at EM level showed reaction mainly with the membranes of the SER. These results suggest that S. aurata saccus vasculosus may produce a PTHrP-like molecule similar to human PTHrP.


Alternate JournalGen. Comp. Endocrinol.
PubMed ID8713647
Grant List / / Wellcome Trust / United Kingdom
CCMAR Authors