|Overexpression of four and a half LIM domains protein 2 promotes epithelial-mesenchymal transition-like phenotype in fish pre-osteoblasts.
|Rafael, MS, Laizé, V, Florindo, C, Ferraresso, S, Bargelloni, L, M. Cancela, L
|Year of Publication
|Alkaline Phosphatase, Animals, Blotting, Western, Cell Line, Cell Proliferation, Collagen, Epithelial-Mesenchymal Transition, Glycosaminoglycans, Real-Time Polymerase Chain Reaction, Sea Bream, Transcription Factors
FHL2 is a multifunctional protein involved in gene transcription regulation and cytoarchitecture modulation that has been recently associated with epithelial-mesenchymal transition (EMT) in colon cancer. Overexpression of FHL2 in a fish pre-osteoblastic cell line promoted cell dedifferentiation and impaired its extracellular matrix mineralization capacity. Cell cultures also acquired a novel three-dimensional structure organization, their proliferation rate was enhanced and gene expression profile was altered in agreement with an EMT-like phenotype upon overexpression of FHL2. Altogether, our results provide additional support to the relevance of FHL2 for cell differentiation and its association with hallmarks of cancer phenotype.