Sulfated polysaccharides in marine sponges: extraction methods and anti-HIV activity. | - CCMAR -

Journal Article

TítuloSulfated polysaccharides in marine sponges: extraction methods and anti-HIV activity.
Publication TypeJournal Article
AuthorsEsteves, AIS, Nicolai, M, Humanes, M, Goncalves, J
Year of Publication2011
JournalMar Drugs
Date Published2011 Jan 24
Palavras-chaveAnimals, Anti-HIV Agents, Aquatic Organisms, Drug Discovery, HIV Fusion Inhibitors, HIV-1, Microbial Sensitivity Tests, Polysaccharides, Porifera, Sulfates

The extraction, fractionation and HIV-1 inhibition potential of polysaccharides extracted from three species of marine sponges, Erylus discophorus, Cliona celata and Stelletta sp., collected in the Northeastern Atlantic, is presented in this work. The anti-HIV activity of 23 polysaccharide pellets and three crude extracts was tested. Crude extracts prepared from Erylus discophorus specimens were all highly active against HIV-1 (90 to 95% inhibition). Cliona celata pellets showed low polysaccharide content (bellow 38.5%) and almost no anti-HIV activity (<10% inhibition). Stelletta sp. pellets, although quite rich in polysaccharide (up to 97.3%), showed only modest bioactivity (<36% HIV-1 inhibition). Erylus discophorus pellets were among the richest in terms of polysaccharide content (up to 98%) and the most active against HIV-1 (up to 95% inhibition). Chromatographic fractionation of the polysaccharide pellet obtained from a specimen of Erylus discophorus (B161) yielded only modestly active fractions. However, we could infer that the active molecule is most probably a high molecular weight sulfated polysaccharide (>2000 kDa), whose mechanism is possibly preventing viral attachment and entry (fusion inhibitor).


Alternate JournalMar Drugs
PubMed ID21339952
PubMed Central IDPMC3039475
CCMAR Authors