Comparative, Adaptive and Functional Skeletal Biology | - CCMAR -

Comparative, Adaptive and Functional Skeletal Biology

Short Title 
BIOSKEL
Description 

Molecular determinants and regulatory mechanisms (transcription and post transcription regulation, alternative and trans-splicing events, RNA editing, epigenetics) affecting gene expression during onset of skeletogenesis and maintenance of bone and cartilage integrity throughout life.
 

Organiza

Main

Main interests

Molecular determinants and regulatory mechanisms (transcription and post transcription regulation, alternative and trans-splicing events, RNA editing, epigenetics) affecting gene expression during onset of skeletogenesis and maintenance of bone and cartilage integrity throughout life.

Special interests

Skeleton-associated vitamin K-dependent proteins (osteocalcin, matrix Gla protein, Gla-rich protein/UCMA) known to affect tissue mineralization in normal and pathological conditions, and also involved in cell differentiation, transformation and tissue integrity; Vitamin K metabolism and its recently identified transcription network of target genes and novel functions; Use of fish as models for human diseases with skeletal phenotype.

Others

Molecular evolution of skeletal proteins; Osteo-adaptation and osteotoxicity associated with environmental conditions; Regeneration of calcified tissues; Nutritional requirements associated to normal and pathological skeletal development; Identification of molecules of marine origin with pro/anti-osteogenic properties; Osteo-immunology and musculoskeletal crosstalk.

SEE ALSO THE BIOSKEL WEBPAGE AT http://bioskel.ccmar.ualg.pt

 

 

 

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Current members (table view)
Coordinator Posição
Leonor Cancela Senior Researcher
Natércia Conceição Researcher
Paulo Jorge Travessa Gavaia Senior Researcher
Vincent Laizé Senior Researcher
Former
Other Past Members 
António Camacho
Filipe Carvalho
Cátia Marques
Cindy Fazenda
Michael Viegas
Marcelo Livramento
Anabela Bensimon-Brito
Gisela Dionisio
Sara Mira
Marta Rafael
João Fidalgo
Jorge Pinto
Ricardo Afonso
Ricardo Leite
Vijayakumar Parameswaran
Brigite Simões
Joana Rosa
Andreia Adrião
Iris Silva
Marta Vitorino
Mariana Santos
Inês Silva
Vânia Roberto
Luis Granadeiro
Daniel António Martins Tiago
João Cardeira
Tatiana Authier
Current members (grid view)
Alessio Carletti's picture
Posição:
PhD Student
Ana Alfaia's picture
Posição:
Débora Varela's picture
Posição:
PhD Student
Gil Sales Marques Martins's picture
Posição:
Research Technician
Helena Isabel Rosa Pinto Caiado's picture
Posição:
PhD Student
Joana T. Rosa's picture
Posição:
Postdoc
Leonor Cancela's picture
Posição:
Senior Researcher
Márcio Alexandre Filipe Simão's picture
Posição:
PhD Student
Marco Tarasco's picture
Posição:
PhD Student
Matthew Castaldi's picture
Posição:
Michele Loiodice's picture
Posição:
MSc Student
Natércia Conceição's picture
Posição:
Researcher
Patrícia Diogo's picture
Posição:
PhD Student
Paulo Jorge Travessa Gavaia's picture
Posição:
Senior Researcher
Sunil Poudel's picture
Posição:
PhD Student
Tatiana Varela's picture
Posição:
Researcher
Vânia P. Roberto's picture
Posição:
Postdoc
Vincent Laizé's picture
Posição:
Senior Researcher
Gallery
Publications
All Publications 
Martins G, Ribeiro L, Candeias‐Mendes A, et al. Reduction of skeletal anomalies in meagre ( Argyrosomus regius , Asso, 1801) through early introduction of inert diet. Aquaculture Research. 2019;50(10):2782 - 2792. doi:10.1111/are.v50.1010.1111/are.14230
Simão M, Gavaia PJ, Camacho A, et al. Intracellular iron uptake is favored in Hfe ‐KO mouse primary chondrocytes mimicking an osteoarthritis‐related phenotype. BioFactors. 2019. doi:10.1002/biof.1520
Diogo P, Martins G, Nogueira R, Marreiros A, Gavaia PJ, Cabrita E. Cryoprotectants synergy improve zebrafish sperm cryopreservation and offspring skeletogenesis. Cryobiology. 2019. doi:10.1016/j.cryobiol.2019.10.001
Oralová V, Rosa JT, Soenens M, et al. Beyond the whole-mount phenotype: high-resolution imaging in fluorescence-based applications on zebrafish. Biology Open. 2019;8(5):bio042374. doi:10.1242/bio.042374
Rosa JT, Oralová V, Larionova D, Eisenhoffer GT, P. Witten E, Huysseune A. Periderm invasion contributes to epithelial formation in the teleost pharynx. Scientific Reports. 2019;9(1). doi:10.1038/s41598-019-46040-y
Fernández I, Fernandes JMO, Roberto VP, et al. Circulating small non-coding RNAs provide new insights into vitamin K nutrition and reproductive physiology in teleost fish. Biochimica et Biophysica Acta (BBA) - General Subjects. 2019;1863(1):39-51. doi:10.1016/j.bbagen.2018.09.017
Carvalho FR, Calado SM, Silva GA, et al. Altered bone microarchitecture in a type 1 diabetes mouse model Ins2 Akita . Journal of Cellular Physiology. 2019;234(6):9338-9350 . doi:10.1002/jcp.27617
Tarasco M, Cardeira J, Viegas M, et al. Anti-osteogenic activity of cadmium in zebrafish. Fishes. 2019;4(1):11. doi:10.3390/fishes4010011
Granadeiro L, Dirks RP, Ortiz-Delgado JB, et al. Warfarin-exposed zebrafish embryos resembles human warfarin embryopathy in a dose and developmental-time dependent manner – From molecular mechanisms to environmental concerns. Ecotoxicology and Environmental Safety. 2019;181:559 - 571. doi:10.1016/j.ecoenv.2019.06.042
Navarro-Guillén C, Dias J, Rocha F, et al. Does a ghrelin stimulus during zebrafish embryonic stage modulate its performance on the long-term?. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology. 2019;228:1 - 8. doi:10.1016/j.cbpa.2018.10.019
Diogo P, Martins G, Eufrásio A, Silva T, Cabrita E, Gavaia P. Selection Criteria of Zebrafish Male Donors for Sperm Cryopreservation. Zebrafish. 2019;16(2):189-196. doi:10.1089/zeb.2018.1660
Martins G, Diogo P, Pinto W, Gavaia PJ. Early Transition to Microdiets Improves Growth, Reproductive Performance and Reduces Skeletal Anomalies in Zebrafish ( Danio rerio ). Zebrafish. 2019;16(3):300-307. doi:10.1089/zeb.2018.1691
Carson MA, Nelson J, M. Cancela L, et al. Correction: Screening for osteogenic activity in extracts from Irish marine organisms: The potential of Ceramium pallidum. PLOS ONE. 2019;14(1):e0211395. doi:10.1371/journal.pone.0211395
Santos JMA, Mendes-Silva L, Afonso V, et al. Exogenous WNT5A and WNT11 proteins rescue CITED2 dysfunction in mouse embryonic stem cells and zebrafish morphants. Cell Death & Disease. 2019;10(8). doi:10.1038/s41419-019-1816-6
Laizé V, Gavaia P, Tarasco M, et al. Osteotoxicity of 3-methylcholanthrene in fish. Ecotoxicology and Environmental Safety. 2018;161:721 - 728. doi:10.1016/j.ecoenv.2018.06.035
Silva IAL, Conceição N, Gagnon E, Brown JP, M. Cancela L, Michou L. Molecular effect of an OPTN common variant associated to Paget's disease of bone. PLOS ONE. 2018;13(5):e0197543. doi:10.1371/journal.pone.0197543.s007
Vitorino M, Cunha N, Conceição N, M. Cancela L. Expression pattern of cdkl5 during zebrafish early development: implications for use as model for atypical Rett syndrome. Molecular Biology Reports. 2018. doi:10.1007/s11033-018-4180-1
Vitorino M, Conceição N, M. Cancela L. Zebrafish (Danio rerio, Hamilton-Buchanan, 1822) as a model to study bone diseases associated with Rett syndrome. Journal of Applied Ichthyology. 2018;34(2):489 - 500. doi:10.1111/jai.13667
Fernández I, Gavaia P, Laizé V, M. Cancela L. Fish as a model to assess chemical toxicity in bone. Aquatic Toxicology. 2018;194:208 - 226. doi:10.1016/j.aquatox.2017.11.015
Silva IAL, Conceição N, Gagnon E, et al. Effect of genetic variants of OPTN in the pathophysiology of Paget's disease of bone. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. 2018;1864(1):143 - 151. doi:10.1016/j.bbadis.2017.10.008
Fernandez I, Gavaia P, Darias M, Gisbert E. Fat-soluble vitamins in fish: A transcriptional tissue-specific crosstalk that remains to be unveiled and characterized. In: Emerging Issues In Fish Larvae Research. Emerging issues in fish larvae research. Springer International Publishing AG; 2018.
Fernández I, Granadeiro L, Darias MJ, Gavaia P, Andree KB, Gisbert E. Solea senegalensis skeletal ossification and gene expression patterns during metamorphosis: New clues on the onset of skeletal deformities during larval to juvenile transition. Aquaculture. 2018;496:153 - 165. doi:10.1016/j.aquaculture.2018.07.022
Gonçalves R, Tarasco M, Schatzmayr D, Gavaia P. Preliminary Evaluation of Moniliformin as a Potential Threat for Teleosts. Fishes. 2018;3(1):4. doi:10.3390/fishes3010004
Rosa J, Cox CJ, M. Cancela L, Laizé V. Identification of a fish short-chain dehydrogenase/reductase associated with bone metabolism. Gene. 2018;645:137 - 145. doi:10.1016/j.gene.2017.12.021
Soares F, Roque A, Gavaia P. Review of the principal diseases affecting cultured meagre ( Argyrosomus regius). Aquaculture Research. 2018. doi:10.1111/are.13613
Roberto VP, Gavaia P, Nunes MJ, Rodrigues E, M. Cancela L, Tiago DM. Evidences for a New Role of miR-214 in Chondrogenesis. Scientific Reports. 2018;8(1). doi:10.1038/s41598-018-21735-w
Roberto VP, Martins G, Pereira A, et al. Insights from dietary supplementation with zinc and strontium on the skeleton of zebrafish, Danio rerio (Hamilton, 1822) larvae: From morphological analysis to osteogenic markers. Journal of Applied Ichthyology. 2018. doi:10.1111/jai.13664
Carson MA, Nelson J, M. Cancela L, et al. Screening for osteogenic activity in extracts from Irish marine organisms: The potential of Ceramium pallidum. Cray JJ, ed. PLOS ONE. 2018;13(11):e0207303. doi:10.1371/journal.pone.0207303.s008
Fazenda C, Martins G, Gavaia P, M. Cancela L, Conceição N. Generation of zebrafish Danio rerio (Hamilton, 1822) transgenic lines overexpressing a heat-shock mediated Gla-rich protein. Journal of Applied Ichthyology. 2018. doi:10.1111/jai.13662
Cardeira J, Laizé V, Martins G, M. Cancela L, Gavaia P. An overview on the teleost bone mechanophysiology. Journal of Applied Ichthyology. 2018. doi:10.1111/jai.13661
Ferreira-Rodríguez N, Fernández I, M. Cancela L, Pardo I. Multibiomarker response shows how native and non-native freshwater bivalves differentially cope with heat-wave events. Aquatic Conservation: Marine and Freshwater Ecosystems. 2018. doi:10.1002/aqc.2884
Diogo P, Martins G, Quinzico I, Nogueira R, Gavaia P, Cabrita E. Electric ultrafreezer (− 150 °C) as an alternative for zebrafish sperm cryopreservation and storage. Fish Physiology and Biochemistry. 2018. doi:10.1007/s10695-018-0500-6
Carson MA, Nelson J, M. Cancela L, et al. Red algal extracts from Plocamium lyngbyanum and Ceramium secundatum stimulate osteogenic activities in vitro and bone growth in zebrafish larvae. Scientific Reports. 2018;8(1). doi:10.1038/s41598-018-26024-0
Fernández I, Ortiz-Delgado JB, Darias MJ, et al. Vitamin A Affects Flatfish Development in a Thyroid Hormone Signaling and Metamorphic Stage Dependent Manner. Frontiers in Physiology. 2017;8. doi:10.3389/fphys.2017.00458
Boglione C, Gavaia P, Koumoundouros G, et al. Skeletal Anomalies in Reared European Fish Larvae and Juveniles. Part 1: Normal and Anomalous Skeletogenic Processes. In: Conceição LEC, Tandler A, eds. Success Factors For Fish Larval Production. Success Factors for Fish Larval Production. Wiley; 2017:219-254. https://books.google.pt/books?id=xV_HrQEACAAJ.
Boglione C, Gisbert E, Gavaia P, et al. Skeletal Anomalies in Reared European Fish Larvae and Juveniles. Part 2:Main Typologies, Occurrences and Causative Factors. In: Conceição LEC, Tandler A, eds. Success Factors For Fish Larval Production. Success Factors for Fish Larval Production. Wiley; 2017:255-330. https://books.google.pt/books?id=xV_HrQEACAAJ.
Riesco MF, Oliveira C, Soares F, Gavaia P, Dinis MT, Cabrita E. Solea senegalensis sperm cryopreservation: New insights on sperm quality. Prunet P, ed. PLOS ONE. 2017;12(10):e0186542. doi:10.1371/journal.pone.0186542.s003
Surget G, Roberto VP, Le Lann K, et al. Marine green macroalgae: a source of natural compounds with mineralogenic and antioxidant activities. Journal of Applied Phycology. 2017;29:575-584. doi:10.1007/s10811-016-0968-3
Carvalho FR, Fernandes AR, M. Cancela L, Gavaia P. Improved regeneration and de novo bone formation in a diabetic zebrafish model treated with Paricalcitol and Cinacalcet. Wound Repair and Regeneration. 2017;86(6):1796–1810. doi:10.1111/wrr.12536
Tarasco M, Laizé V, Cardeira J, M. Cancela L, Gavaia P. The zebrafish operculum: A powerful system to assess osteogenic bioactivities of molecules with pharmacological and toxicological relevance. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 2017;197:45 - 52. doi:10.1016/j.cbpc.2017.04.006
Marques C, Roberto VP, Granadeiro L, et al. The xenobiotic sensor PXR in a marine flatfish species ( Solea senegalensis ): Gene expression patterns and its regulation under different physiological conditions. Marine Environmental Research. 2017;130:187-199. doi:10.1016/j.marenvres.2017.07.021
Marques CL, M. Cancela L, Laizé V. Transcriptional regulation of gilthead seabream bone morphogenetic protein (BMP) 2 gene by bone- and cartilage-related transcription factors. Gene. 2016;576(1 Pt 2):229-236. doi:10.1016/j.gene.2015.10.005
Tiago DM, Conceição N, Caiado H, Laizé V, M. Cancela L. Matrix Gla protein repression by miR-155 promotes oncogenic signals in breast cancer MCF-7 cells. FEBS Letters. 2016;590(8):1234-1241. doi:10.1002/1873-3468.12155
Marques CL, Fernández I, Viegas MN, et al. Comparative analysis of zebrafish bone morphogenetic proteins 2, 4 and 16: molecular and evolutionary perspectives. Cellular and Molecular Life Sciences. 2016;73(4):841-857. doi:10.1007/s00018-015-2024-x
Bensimon-Brito A, Cardeira J, Dionísio G, Huysseune A, M. Cancela L, Witten PE. Revisiting in vivo staining with alizarin red S--a valuable approach to analyse zebrafish skeletal mineralization during development and regeneration. BMC Dev Biol. 2016;16:2. doi:10.1186/s12861-016-0102-4
Adrião A, Conceição N, M. Cancela L. MEF2C orthologues from zebrafish: Evolution, expression and promoter regulation. Arch Biochem Biophys. 2016;591:43-56. doi:10.1016/j.abb.2015.12.004
Rosa J, Tiago DM, Marques CL, et al. Central role of betaine–homocysteine S-methyltransferase 3 in chondral ossification and evidence for sub-functionalization in neoteleost fish. Biochimica et Biophysica Acta (BBA) - General Subjects. 2016;1860(7):1373-1387. doi:10.1016/j.bbagen.2016.03.034
Correia E, Conceição N, M. Cancela L, Belo JA. Matrix Gla Protein expression pattern in the early avian embryo. Int J Dev Biol. 2016;60(1-3):71-6. doi:10.1387/ijdb.150365jb
Divisato G, Formicola D, Esposito T, et al. ZNF687 Mutations in Severe Paget Disease of Bone Associated with Giant Cell Tumor. Am J Hum Genet. 2016;98(2):275-86. doi:10.1016/j.ajhg.2015.12.016
Medeiros MCR, Mestre A, Inácio P, et al. An electrical method to measure low-frequency collective and synchronized cell activity using extracellular electrodes. Sensing and Bio-Sensing Research. 2016;10:1 - 8. doi:10.1016/j.sbsr.2016.06.002
Cardeira J, Gavaia P, Fernández I, et al. Quantitative assessment of the regenerative and mineralogenic performances of the zebrafish caudal fin. Scientific Reports. 2016;6:39191. doi:10.1038/srep39191
Ferreira-Rodríguez N, Fernández I, Varandas S, Cortes R, M. Cancela L, Pardo I. The role of calcium concentration in the invasive capacity of Corbicula fluminea in crystalline basins. Science of The Total Environment. 2016. doi:10.1016/j.scitotenv.2016.12.100
I F, PJ G. Vitamin K, much more than blood clotting: Roles, metabolism and specific requirements. A mini-review. . In: Avances En Nutrición Acuícola Xiii. Cruz Suárez LE, Ricque Marie D, Tapia Salazar M, Nieto López MG, Villarreal Cavazos DA, Gamboa J, Rivas Vega ME, Miranda A Avances en Nutrición Acuícola XIII. ; 2016.
Camacho A, Funck-Brentano T, Simão M, et al. Effect of C282Y genotype on self-reported musculoskeletal complications in hereditary hemochromatosis. PLoS One. 2015;10(3):e0122817. doi:10.1371/journal.pone.0122817
Fernández I, López-Joven C, Andree KB, Roque A, Gisbert E. Vitamin A supplementation enhances Senegalese sole (Solea senegalensis) early juvenile's immunocompetence: New insights on potential underlying pathways. Fish & Shellfish Immunology. 2015;46(2):703 - 709. doi:10.1016/j.fsi.2015.08.007
Silva IAL, Conceição N. Cloning, characterization and analysis of the 5′ regulatory region of zebrafish xpd gene. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. 2015;185:47 - 53. doi:10.1016/j.cbpb.2015.04.003
Rocha F, Dias J, Engrola S, et al. Glucose metabolism and gene expression in juvenile zebrafish (Danio rerio) challenged with a high carbohydrate diet: effects of an acute glucose stimulus during late embryonic life. British Journal of Nutrition. 2015;113(03):403 - 413. doi:10.1017/S0007114514003869
Salas-Leiton E, Dias J, Gavaia P, Amoedo A, Valente LMP. Optimization of phosphorus content in high plant protein practical diets for Senegalese sole ( Solea senegalensis, Kaup 1858) juveniles: influence on growth performance and composition of whole body and vertebrae. Aquaculture Nutrition. 2015:n/a - n/a. doi:10.1111/anu.12354
Rodrigues ET, Pardal MÂ, Laizé V, M. Cancela L, Oliveira PJ, Serafim TL. Cardiomyocyte H9c2 cells present a valuable alternative to fish lethal testing for azoxystrobin. Environ Pollut. 2015;206:619-26. doi:10.1016/j.envpol.2015.08.026
Diogo P, Martins G, Gavaia P, et al. Assessment of nutritional supplementation in phospholipids on the reproductive performance of zebrafish, Danio rerio (Hamilton, 1822). Journal of Applied Ichthyology. 2015;31:3 - 9. doi:10.1111/jai.2015.31.issue-S110.1111/jai.12733
Fazenda C, Conceição N, M. Cancela L. Transcription factors from Sox family regulate expression of zebrafish Gla-rich protein 2 gene. Gene. 2015;572(1):57-62. doi:10.1016/j.gene.2015.06.079
Fernández I, Vijayakumar P, Marques C, M. Cancela L, Gavaia P, Laizé V. Zebrafish vitamin K epoxide reductases: expression in vivo, along extracellular matrix mineralization and under phylloquinone and warfarin in vitro exposure. Fish Physiology and Biochemistry. 2015;41(3):745-759. doi:10.1007/s10695-015-0043-z
Cardeira J, Mendes AC, Pousão-Ferreira P, M. Cancela L, Gavaia P. Micro-anatomical characterization of vertebral curvatures in Senegalese sole Solea senegalensis. J Fish Biol. 2015;86(6):1796-810. doi:10.1111/jfb.12686
Roberto VP, Tiago DM, Gautvik K, M. Cancela L. Evidence for the conservation of miR-223 in zebrafish (Danio rerio): Implications for function. Gene. 2015;566(1):54-62. doi:10.1016/j.gene.2015.04.022
Simões B, Conceição N, Matias AC, Bragança J, Kelsh RN, M. Cancela L. Molecular characterization of cbfβ gene and identification of new transcription variants: implications for function. Arch Biochem Biophys. 2015;567:1-12. doi:10.1016/j.abb.2014.12.023
Fernández I, Cardeira J, Granadeiro L, Gavaia P, Laizé V, M. Cancela L. The use of in vivo imaging tools in zebrafish for drug discovery for bone development and homeostasis. Laboratory Animals. 2015;49:26 - 94. doi:10.1177/0023677215609551
Beirão J, Soares F, Pousão-Ferreira P, et al. The effect of enriched diets on Solea senegalensis sperm quality. Aquaculture. 2015;435:187 - 194. doi:10.1016/j.aquaculture.2014.09.025
Laizé V, Gavaia P, M. Cancela L. Fish: a suitable system to model human bone disorders and discover drugs with osteogenic or osteotoxic activities. Drug Discovery Today: Disease Models. 2014;13:29-37. doi:10.1016/j.ddmod.2014.08.001
Richard N, Fernández I, Wulff T, et al. Dietary supplementation with vitamin k affects transcriptome and proteome of Senegalese sole, improving larval performance and quality. Mar Biotechnol (NY). 2014;16(5):522-37. doi:10.1007/s10126-014-9571-2
Fernández I, Santos A, M. Cancela L, Laizé V, Gavaia P. Warfarin, a potential pollutant in aquatic environment acting through Pxr signaling pathway and γ-glutamyl carboxylation of vitamin K-dependent proteins. Environmental Pollution. 2014;194:86-95. doi:10.1016/j.envpol.2014.07.015
Cavaco S, Williamson MK, Rosa J, et al. Teleost fish osteocalcin 1 and 2 share the ability to bind the calcium mineral phase. Fish Physiol Biochem. 2014;40(3):731-8. doi:10.1007/s10695-013-9880-9
Tiago DM, Marques CL, Roberto VP, M. Cancela L, Laizé V. Mir-20a regulates in vitro mineralization and BMP signaling pathway by targeting BMP-2 transcript in fish. Arch Biochem Biophys. 2014;543:23-30. doi:10.1016/j.abb.2013.12.009
Rocha F, Dias J, Engrola S, et al. Glucose overload in yolk has little effect on the long-term modulation of carbohydrate metabolic genes in zebrafish (Danio rerio). J Exp Biol. 2014;217(Pt 7):1139-49. doi:10.1242/jeb.095463
Marques CL, Fernández I, Rosa J, Viegas MN, M. Cancela L, Laizé V. Spatiotemporal expression and retinoic acid regulation of bone morphogenetic proteins 2, 4 and 16 in Senegalese sole. Journal of Applied Ichthyology. 2014;30(4):713-720. doi:10.1111/jai.2014.30.issue-410.1111/jai.12539
M. Cancela L, Laizé V, Conceição N. Matrix Gla protein and osteocalcin: from gene duplication to neofunctionalization. Arch Biochem Biophys. 2014;561:56-63. doi:10.1016/j.abb.2014.07.020
F S, I F, B C, PJ G. Non-infectious disorders of warm water fish . In: Diseases And Disorders Of Finfish In Cage Culture. CABI publishing. Diseases and Disorders of Finfish in Cage Culture. ; 2014.
Rosa JT, M. Cancela L, Laizé V. Ets1 regulates the transcription of a cartilage-specific S100 protein in gilthead seabream. Journal of Applied Ichthyology. 2014;30(4):707-712. doi:10.1111/jai.2014.30.issue-410.1111/jai.12534
Cabrita E, Martínez-Páramo S, Gavaia P, et al. Factors enhancing fish sperm quality and emerging tools for sperm analysis. Aquaculture. 2014;432:389 - 401. doi:10.1016/j.aquaculture.2014.04.034
E G, I F, N V, MJ D, JL Z-I, A E. Larval development. In: Biology Of European Seabass. Science Publishers, Hampshire, USA. Biology of European Seabass. ; 2014.
Fernández I, Tiago DM, Laizé V, M. Cancela L, Gisbert E. Retinoic acid differentially affects in vitro proliferation, differentiation and mineralization of two fish bone-derived cell lines: Different gene expression of nuclear receptors and ECM proteins. Journal of Steroid Biochemistry and Molecular Biology. 2014;140:34-43. doi:10.1016/j.jsbmb.2013.11.012
Simões B, Conceição N, Kelsh RN, M. Cancela L. Identification of cis -regulatory elements in the upstream regions of zebrafish runx3 through in silico analysis: implications for function. Journal of Applied Ichthyology. 2014;30(4):661 - 670. doi:10.1111/jai.2014.30.issue-410.1111/jai.12536
Roberto VP, Tiago DM, Silva IAL, M. Cancela L. MiR-29a is an enhancer of mineral deposition in bone-derived systems. Arch Biochem Biophys. 2014;564:173-83. doi:10.1016/j.abb.2014.09.006
Mateus M, Gamboa A, Ribeiro L, et al. Does copepods influence dusky grouper (Epinephelus marginatus) early development?. Frontiers in Marine Science. 2014;1. doi:10.3389/conf.FMARS.2014.02.00024
Soares F, Monzón I, Costas B, Gavaia P. Non-infectious Disorders of Warm water Fish Chapter 7. . In: Diseases And Disorders Of Finfish In Cage Culture. 2nd ed. Diseases and Disorders of Finfish in Cage Culture. ; 2014.
Ortiz-Delgado JB, Fernández I, Sarasquete C, Gisbert E. Normal and histopathological organization of the opercular bone and vertebrae in gilthead sea bream Sparus aurata. Aquatic Biology. 2014;21(1):67 - 84. doi:10.3354/ab00568
Silva IAL, Cox CJ, Leite RB, M. Cancela L, Conceição N. Evolutionary conservation of TFIIH subunits: implications for the use of zebrafish as a model to study TFIIH function and regulation. Comp Biochem Physiol B Biochem Mol Biol. 2014;172-173:9-20. doi:10.1016/j.cbpb.2014.03.007
Silva IAL, Conceição N, Michou L, M. Cancela L. Can zebrafish be a valid model to study Paget's disease of bone?. Journal of Applied Ichthyology. 2014;30(4):678 - 688. doi:10.1111/jai.2014.30.issue-410.1111/jai.12523
Vijayakumar P, Laizé V, Cardeira J, Trindade M, M. Cancela L. Development of an in vitro cell system from zebrafish suitable to study bone cell differentiation and extracellular matrix mineralization. Zebrafish. 2013;10(4):500-9. doi:10.1089/zeb.2012.0833
Viegas CSB, Simes DC, Williamson MK, et al. Sturgeon osteocalcin shares structural features with matrix Gla protein: evolutionary relationship and functional implications. J Biol Chem. 2013;288(39):27801-11. doi:10.1074/jbc.M113.450213
Araujo NCP, Afonso R, Bringela A, M. Cancela L, Cristiano MLS, Leite RB. Peroxides with antiplasmodial activity inhibit proliferation of Perkinsus olseni, the causative agent of Perkinsosis in bivalves. Parasitol Int. 2013;62(6):575-82. doi:10.1016/j.parint.2013.06.010
Conceição N, Viegas M, Fidalgo J, M. Cancela L. Development and characterization of Xl1, a Xenopus laevis chondrocyte-like cell culture. Mol Cell Biochem. 2013;373(1-2):41-51. doi:10.1007/s11010-012-1473-x
Pacchiarini T, Cross I, Leite RB, et al. Solea senegalensis vasa transcripts: molecular characterisation, tissue distribution and developmental expression profiles. Reprod Fertil Dev. 2013;25(4):646-60. doi:10.1071/RD11240
Martínez-Páramo S, Diogo P, Dinis MT, Soares F, Sarasquete C, Cabrita E. Effect of two sulfur-containing amino acids, taurine and hypotaurine in European sea bass (Dicentrarchus labrax) sperm cryopreservation. Cryobiology. 2013;66(3):333-8. doi:10.1016/j.cryobiol.2013.04.001
Cunha ME, Ré P, Quental-Ferreira H, Gavaia P, Pousão-Ferreira P. Larval and juvenile development of dusky grouper Epinephelus marginatus reared in mesocosms. J Fish Biol. 2013;83(3):448-65. doi:10.1111/jfb.12180
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Adrião A, Conceicão N, Gavaia P, M. Cancela L. Zebrafish as a model to study craniofacial phenotypes related to human MEF2C mutations. European Journal of Clinical Investigation. 2013;43:97 - 106. doi:10.1111/eci.2013.43.issue-s110.1111/eci.12088
Leite RB, Milan M, Coppe A, et al. mRNA-Seq and microarray development for the Grooved Carpet shell clam, Ruditapes decussatus: a functional approach to unravel host-parasite interaction. BMC Genomics. 2013;14:741. doi:10.1186/1471-2164-14-741
Simão M, Gavaia P, Pinto J, Korng E, M. Cancela L. Establishing an in vitro system to study chondrocyte phenotypes associated to human hereditary hemochromatosis and identify molecular players involved in chondrocyte related iron metabolism. Bone Abstracts. 2013. doi:10.1530/boneabs.1.PP262
Darias MJ, Andree KB, Boglino A, Fernández I, Estevez A, Gisbert E. Coordinated Regulation of Chromatophore Differentiation and Melanogenesis during the Ontogeny of Skin Pigmentation of Solea senegalensis (Kaup, 1858). PLoS ONE. 2013;8(5):e63005. doi:10.1371/journal.pone.0063005
Conceição N, Fazenda C, M. Cancela L. Comparative gene promoter analysis: an in silico strategy to identify candidate regulatory factors for Gla Rich Protein. Journal of Applied Ichthyology. 2012;28(3):372 - 376. doi:10.1111/j.1439-0426.2012.02001.x
Martínez-Páramo S, Diogo P, Dinis MT, Herráez MP, Sarasquete C, Cabrita E. Incorporation of ascorbic acid and α-tocopherol to the extender media to enhance antioxidant system of cryopreserved sea bass sperm. Theriogenology. 2012;77(6):1129-36. doi:10.1016/j.theriogenology.2011.10.017
Gisbert E, Skalli A, Fernández I, Kotzamanis Y, Zambonino-Infante JLuis, Fabregat R. Protein hydrolysates from yeast and pig blood as alternative raw materials in microdiets for gilthead sea bream (Sparus aurata) larvae. Aquaculture. 2012;338-341:96 - 104. doi:10.1016/j.aquaculture.2012.01.007
Martínez-Páramo S, Diogo P, Dinis MT, Herráez MP, Sarasquete C, Cabrita E. Sea bass sperm freezability is influenced by motility variables and membrane lipid composition but not by membrane integrity and lipid peroxidation. Anim Reprod Sci. 2012;131(3-4):211-8. doi:10.1016/j.anireprosci.2012.03.008
Fazenda C, Silva IAL, M. Cancela L, Conceição N. Molecular characterization of two paralog genes encoding Gla-rich protein (Grp) in zebrafish. Journal of Applied Ichthyology. 2012;28(3):377 - 381. doi:10.1111/j.1439-0426.2012.02004.x
Parameswaran V, Laizé V, Gavaia P, M. Cancela L. ESSA1 embryonic stem like cells from gilthead seabream: a new tool to study mesenchymal cell lineage differentiation in fish. Differentiation. 2012;84(3):240-51. doi:10.1016/j.diff.2012.07.004
Bensimon-Brito A, Cardeira J, M. Cancela L, Huysseune A, Witten PEckhard. Distinct patterns of notochord mineralization in zebrafish coincide with the localization of Osteocalcin isoform 1 during early vertebral centra formation. BMC Dev Biol. 2012;12:28. doi:10.1186/1471-213X-12-28
Bensimon-Brito A, M. Cancela L, Huysseune A, Witten PEckhard. Vestiges, rudiments and fusion events: the zebrafish caudal fin endoskeleton in an evo-devo perspective. Evol Dev. 2012;14(1):116-27. doi:10.1111/j.1525-142X.2011.00526.x
Viegas MN, Dias J, M. Cancela L, Laizé V. Polyunsaturated fatty acids regulate cell proliferation, extracellular matrix mineralization and gene expression in a gilthead seabream skeletal cell line. Journal of Applied Ichthyology. 2012;28(3):427-432. doi:10.1111/j.1439-0426.2012.01994.x
Michou L, Conceição N, Morissette J, et al. Genetic association study of UCMA/GRP and OPTN genes (PDB6 locus) with Paget's disease of bone. Bone. 2012;51(4):720-8. doi:10.1016/j.bone.2012.06.028
Dionísio G, Campos C, Valente LMP, Conceição LEC, M. Cancela L, Gavaia P. Effect of egg incubation temperature on the occurrence of skeletal deformities in Solea senegalensis. Journal of Applied Ichthyology. 2012;28(3):471 - 476. doi:10.1111/j.1439-0426.2012.01996.x
Fernández I, Ortiz-Delgado JB, Sarasquete C, Gisbert E. Vitamin A effects on vertebral bone tissue homeostasis in gilthead sea bream (Sparus aurata) juveniles. Journal of Applied Ichthyology. 2012;28(3):419 - 426. doi:10.1111/j.1439-0426.2012.01997.x
M. Cancela L, Conceição N, Laizé V. Gla-rich protein, a new player in tissue calcification?. Adv Nutr. 2012;3(2):174-81. doi:10.3945/an.111.001685
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Rafael MS, Laizé V, Florindo C, Ferraresso S, Bargelloni L, M. Cancela L. Overexpression of four and a half LIM domains protein 2 promotes epithelial-mesenchymal transition-like phenotype in fish pre-osteoblasts. Biochimie. 2012;94(5):1128-34. doi:10.1016/j.biochi.2012.01.013
Silva IAL, M. Cancela L, Conceição N. Molecular cloning and expression analysis of xpd from zebrafish (Danio rerio). Mol Biol Rep. 2012;39(5):5339-48. doi:10.1007/s11033-011-1333-x
Cardeira J, Vallés R, Dionísio G, et al. Osteology of the axial and appendicular skeletons of the meagre Argyrosomus regius (Sciaenidae) and early skeletal development at two rearing facilities. Journal of Applied Ichthyology. 2012;28(3):464 - 470. doi:10.1111/j.1439-0426.2012.01979.x
Cardeira J, Bensimon-Brito A, Pousão-Ferreira P, M. Cancela L, Gavaia P. Lordotic-kyphotic vertebrae develop ectopic cartilage-like tissue in Senegalese sole (Solea senegalensis). Journal of Applied Ichthyology. 2012;28(3):460 - 463. doi:10.1111/j.1439-0426.2012.01978.x
Richard N, Gavaia P, de Vareilles M, et al. Farm Animal Proteomicstwo-Dimensional Proteomics As A Tool To Evaluate Nutritional Effects In Farmed Fish. (Rodrigues P, Eckersall D, de Almeida A, eds.). Wageningen: Wageningen Academic Publishers; 2012:156 - 158. doi:10.3920/978-90-8686-751-6_37
Rafael MS, Laizé V, Bensimon-Brito A, Leite RB, Schüle R, M. Cancela L. Four-and-a-half LIM domains protein 2 (FHL2) is associated with the development of craniofacial musculature in the teleost fish Sparus aurata. Cell Mol Life Sci. 2012;69(3):423-34. doi:10.1007/s00018-011-0754-y
Martínez-Páramo S, Diogo P, Beirão J, Dinis MT, Cabrita E. Sperm lipid peroxidation is correlated with differences in sperm quality during the reproductive season in precocious European sea bass (Dicentrarchus labrax) males. Aquaculture. 2012;358-359:246 - 252. doi:10.1016/j.aquaculture.2012.06.010
Fonseca VG, Rosa J, Laizé V, Gavaia P, M. Cancela L. Identification of a new cartilage-specific S100-like protein up-regulated during endo/perichondral mineralization in gilthead seabream. Gene Expr Patterns. 2011;11(7):448-55. doi:10.1016/j.gep.2011.07.003
Fernández I, Gisbert E. The effect of vitamin A on flatfish development and skeletogenesis: A review. Aquaculture. 2011;315(1-2):34 - 48. doi:10.1016/j.aquaculture.2010.11.025
Fernández I, Darias M, Andree KB, Mazurais D, Zambonino-Infante J, Gisbert E. Coordinated gene expression during gilthead sea bream skeletogenesis and its disruption by nutritional hypervitaminosis A. BMC Developmental Biology. 2011;11(1):7. doi:10.1186/1471-213X-11-7
Treviño L, Alvarez-González CA, Perales-García N, et al. A histological study of the organogenesis of the digestive system in bay snook Petenia splendida Günther, 1862 from hatching to the juvenile stage. Journal of Applied Ichthyology. 2011;27(1):73 - 82. doi:10.1111/jai.2011.27.issue-110.1111/j.1439-0426.2010.01608.x
Gisbert E, Fernández I, Alvarez-González CA. Prolonged feed deprivation does not permanently compromise digestive function in migrating European glass eels Anguilla anguilla. Journal of Fish Biology. 2011;78(2):580 - 592. doi:10.1111/jfb.2011.78.issue-210.1111/j.1095-8649.2010.02879.x
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Tiago DM, Laizé V, Bargelloni L, Ferraresso S, Romualdi C, M. Cancela L. Global analysis of gene expression in mineralizing fish vertebra-derived cell lines: new insights into anti-mineralogenic effect of vanadate. BMC Genomics. 2011;12:310. doi:10.1186/1471-2164-12-310
Tiago DM, M. Cancela L, Laizé V. Proliferative and mineralogenic effects of insulin, IGF-1, and vanadate in fish osteoblast-like cells. J Bone Miner Metab. 2011;29(3):377-82. doi:10.1007/s00774-010-0243-7
Milan M, Coppe A, Reinhardt R, et al. Transcriptome sequencing and microarray development for the Manila clam, Ruditapes philippinarum: genomic tools for environmental monitoring. BMC Genomics. 2011;12:234. doi:10.1186/1471-2164-12-234
Cabrita E, Ma S, Diogo P, Martínez-Páramo S, Sarasquete C, Dinis MT. The influence of certain aminoacids and vitamins on post-thaw fish sperm motility, viability and DNA fragmentation. Anim Reprod Sci. 2011;125(1-4):189-95. doi:10.1016/j.anireprosci.2011.03.003
Louro B, Passos ALúcia, Souche EL, et al. Gilthead sea bream (Sparus auratus) and European sea bass (Dicentrarchus labrax) expressed sequence tags: Characterization, tissue-specific expression and gene markers. Mar Genomics. 2010;3(3-4):179-91. doi:10.1016/j.margen.2010.09.005
Rafael MS, Marques CL, Parameswaran V, M. Cancela L, Laizé V. Fish bone-derived cell lines: an alternative in vitro cell system to study bone biology. Journal of Applied Ichthyology. 2010;26(2):230-234. doi:10.1111/jai.2010.26.issue-210.1111/j.1439-0426.2010.01411.x
Dâmaso-Rodrigues MLuísa, Pousão-Ferreira P, Ribeiro L, et al. Lack of essential fatty acids in live feed during larval and post-larval rearing: effect on the performance of juvenile Solea senegalensis. Aquaculture International. 2010;18(5):741 - 757. doi:10.1007/s10499-009-9296-9
Viegas CSB, Conceição N, Fazenda C, Simes DC, M. Cancela L. Expression of Gla-rich protein (GRP) in newly developed cartilage-derived cell cultures from sturgeon ( Acipenser naccarii ). Journal of Applied Ichthyology. 2010;26(2):214 - 218. doi:10.1111/jai.2010.26.issue-210.1111/j.1439-0426.2010.01408.x
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Cunha ME, Quental-Fereira H, Boglione C, , Gavaia P, Pousão-Fereira P. Rearing fish larvae for extensive and semi-intensive aquaculture - the “natural” mesocosms system. Aquaculture Europe 35 (2): 23-26. 2010.
Fernández I, Gisbert E. Senegalese sole bone tissue originated from chondral ossification is more sensitive than dermal bone to high vitamin A content in enriched Artemia. Journal of Applied Ichthyology. 2010;26(2):344 - 349. doi:10.1111/jai.2010.26.issue-210.1111/j.1439-0426.2010.01432.x
Conceição LEC, Aragão C, Richard N, et al. Novel methodologies in marine fish larval nutrition. Fish Physiology and Biochemistry. 2010;36(1):1 - 16. doi:10.1007/s10695-009-9373-z
Simão MF, Leite RB, Rocha C, M. Cancela L. Changes in bioturbation of iron biogeochemistry and in molecular response of the clam Ruditapes decussates upon Perkinsus olseni infection. Arch Environ Contam Toxicol. 2010;59(3):433-43. doi:10.1007/s00244-010-9490-9
Conceição N, Cox CJ, Simões B, Viegas M, M. Cancela L. Comparative promoter analysis and its application to the identification of candidate regulatory factors of cartilage-expressed genes. Journal of Applied Ichthyology. 2010;26(2):245 - 250. doi:10.1111/jai.2010.26.issue-210.1111/j.1439-0426.2010.01415.x
Fazenda C, Simões B, Kelsh RN, M. Cancela L, Conceição N. Dual transcriptional regulation by runx2 of matrix Gla protein in Xenopus laevis. Gene. 2010;450(1-2):94-102. doi:10.1016/j.gene.2009.10.007
Diogo P, Soares F, Dinis MT, Cabrita E. The influence of ovarian fluid on Solea senegalensis sperm motility. Journal of Applied Ichthyology. 2010;26(5):690 - 695. doi:10.1111/jai.2010.26.issue-510.1111/j.1439-0426.2010.01554.x
Rosa J, Tiago DM, Dias J, M. Cancela L, Laizé V. Serum-specific stimulation of proliferation and mineralization of fish bone-derived cells. Journal of Applied Ichthyology. 2010;26(2):251 - 256. doi:10.1111/jai.2010.26.issue-210.1111/j.1439-0426.2010.01414.x
Engrola S, Figueira L, Conceição LEC, Gavaia P, Ribeiro L, Dinis MT. Co-feeding in Senegalese sole larvae with inert diet from mouth opening promotes growth at weaning. Aquaculture. 2009;288(3-4):264 - 272. doi:10.1016/j.aquaculture.2008.12.010
Gavaia P, Domingues S, Engrola S, et al. Comparing skeletal development of wild and hatchery-reared Senegalese sole ( Solea senegalensis , Kaup 1858): evaluation in larval and postlarval stages. Aquaculture Research. 2009;40(14):1585 - 1593. doi:10.1111/are.2009.40.issue-1410.1111/j.1365-2109.2009.02258.x
Conceição LEC, Aragão C, Richard N, et al. Avanços recentes em nutrição de larvas de peixes. Revista Brasileira de Zootecnia. 2009;38(spe):26 - 35. doi:10.1590/S1516-35982009001300003
Fernández I, Pimentel MS, Ortiz-Delgado JB, et al. Effect of dietary vitamin A on Senegalese sole (Solea senegalensis) skeletogenesis and larval quality. Aquaculture. 2009;295(3-4):250 - 265. doi:10.1016/j.aquaculture.2009.06.046
Gisbert E, Giménez G, Fernández I, Kotzamanis Y, Estevez A. Development of digestive enzymes in common dentex Dentex dentex during early ontogeny. Aquaculture. 2009;287(3-4):381 - 387. doi:10.1016/j.aquaculture.2008.10.039
Roberto VP, Cavaco S, Viegas CSB, et al. Matrix Gla protein in turbot (Scophthalmus maximus): Gene expression analysis and identification of sites of protein accumulation. Aquaculture. 2009;294(3-4):202 - 211. doi:10.1016/j.aquaculture.2009.06.020
Laizé V, Tiago DM, Aureliano M, M. Cancela L. New insights into mineralogenic effects of vanadate. Cell Mol Life Sci. 2009;66(24):3831-6. doi:10.1007/s00018-009-0137-9
Viegas CSB, Cavaco S, Neves PL, et al. Gla-rich protein is a novel vitamin K-dependent protein present in serum that accumulates at sites of pathological calcifications. Am J Pathol. 2009;175(6):2288-98. doi:10.2353/ajpath.2009.090474
Ascenso RMT, Leite RB, Afonso R, M. Cancela L. Expression pattern of Perkinsus olseni genes in response to bivalves with different susceptibility to perkinsosis. J Fish Dis. 2009;32(7):633-6. doi:10.1111/j.1365-2761.2009.01039.x
Leite RB, Brito AB, M. Cancela L. An oxygen molecular sensor, the HIF prolyl 4-hydroxylase, in the marine protist Perkinsus olseni. Protist. 2008;159(3):355-68. doi:10.1016/j.protis.2008.03.002
Elandalloussi LM, Leite RB, Rodrigues P, Afonso R, M. Cancela L. Effect of the herbicide Roundup on Perkinsus olseni in vitro proliferation and in vivo survival when infecting a permissive host, the clam Ruditapes decussatus. Bull Environ Contam Toxicol. 2008;80(6):512-5. doi:10.1007/s00128-008-9412-y
Coesel SNicole, Baumgartner ACordeiro, Teles LMarlene, et al. Nutrient limitation is the main regulatory factor for carotenoid accumulation and for Psy and Pds steady state transcript levels in Dunaliella salina (Chlorophyta) exposed to high light and salt stress. Mar Biotechnol (NY). 2008;10(5):602-11. doi:10.1007/s10126-008-9100-2
Tanguy A, Bierne N, Saavedra C, et al. Increasing genomic information in bivalves through new EST collections in four species: development of new genetic markers for environmental studies and genome evolution. Gene. 2008;408(1-2):27-36. doi:10.1016/j.gene.2007.10.021
Tiago DM, Laizé V, M. Cancela L, Aureliano M. Impairment of mineralization by metavanadate and decavanadate solutions in a fish bone-derived cell line. Cell Biol Toxicol. 2008;24(3):253-63. doi:10.1007/s10565-007-9034-x
Conceição N, Laizé V, Simões B, Pombinho AR, M. Cancela L. Retinoic acid is a negative regulator of matrix Gla protein gene expression in teleost fish Sparus aurata. Biochim Biophys Acta. 2008;1779(1):28-39. doi:10.1016/j.bbagrm.2007.11.003
Fernández I, Hontoria F, Ortiz-Delgado JB, et al. Larval performance and skeletal deformities in farmed gilthead sea bream (Sparus aurata) fed with graded levels of Vitamin A enriched rotifers (Brachionus plicatilis). Aquaculture. 2008;283(1-4):102 - 115. doi:10.1016/j.aquaculture.2008.06.037
Tiago DM, M. Cancela L, Aureliano M, Laizé V. Vanadate proliferative and anti-mineralogenic effects are mediated by MAPK and PI-3K/Ras/Erk pathways in a fish chondrocyte cell line. FEBS Lett. 2008;582(9):1381-5. doi:10.1016/j.febslet.2008.03.025
Tiago DM, Laizé V, M. Cancela L. Alternatively spliced transcripts of Sparus aurata insulin-like growth factor 1 are differentially expressed in adult tissues and during early development. Gen Comp Endocrinol. 2008;157(2):107-15. doi:10.1016/j.ygcen.2008.04.006
Viegas CSB, Simes DC, Laizé V, Williamson MK, Price PA, M. Cancela L. Gla-rich protein (GRP), a new vitamin K-dependent protein identified from sturgeon cartilage and highly conserved in vertebrates. J Biol Chem. 2008;283(52):36655-64. doi:10.1074/jbc.M802761200
Marques CL, Rafael MS, M. Cancela L, Laizé V. Establishment of primary cell cultures from fish calcified tissues. Cytotechnology. 2007;55(1):9-13. doi:10.1007/s10616-007-9098-8
Stefanni S, Porteiro FM, Bettencourt R, Gavaia P, Santos RS. Molecular insights indicate that Pachycara thermophilum (Geistdoerfer, 1994) and P. saldanhai (Biscoito and Almeida, 2004) (Perciformes: Zoarcidae) from the Mid-Atlantic Ridge are synonymous species. Molecular Phylogenetics and Evolution. 2007;45(1):423 - 426. doi:10.1016/j.ympev.2007.02.001
Rønnestad I, Gavaia P, Viegas CSB, et al. Oligopeptide transporter PepT1 in Atlantic cod (Gadus morhua L.): cloning, tissue expression and comparative aspects. J Exp Biol. 2007;210(Pt 22):3883-96. doi:10.1242/jeb.007898
Kohli V, Robles V, M. Cancela L, Acker JP, Waskiewicz AJ, Elezzabi AY. An alternative method for delivering exogenous material into developing zebrafish embryos. Biotechnol Bioeng. 2007;98(6):1230-41. doi:10.1002/bit.21564
Robles V, Barbosa V, Herráez MP, Martínez-Páramo S, M. Cancela L. The antifreeze protein type I (AFP I) increases seabream (Sparus aurata) embryos tolerance to low temperatures. Theriogenology. 2007;68(2):284-9. doi:10.1016/j.theriogenology.2007.05.003
Stefanni S, Porteiro FM, Bettencourt R, Gavaia P, Santos RS. Molecular insights indicate that Pachycara thermophilum () and P. saldanhai () (Perciformes: Zoarcidae) from the Mid-Atlantic Ridge are synonymous species. Mol Phylogenet Evol. 2007;45(1):423-6. doi:10.1016/j.ympev.2007.02.001
Fonseca VG, Laizé V, Valente M, M. Cancela L. Identification of an osteopontin-like protein in fish associated with mineral formation. FEBS J. 2007;274(17):4428-39. doi:10.1111/j.1742-4658.2007.05972.x
Pittman K, Rønnestad I, Gavaia P, et al. Fish Larval Research: a tool for sustainable food production and understanding environmental impacts on developing organisms. Aquaculture Europe . 2007;32(5).
Brito ABB, M. Cancela L, Gavaia P. Expression of osteonectin correlates with levels of fin regeneration in zebrafish (Danio rerio). FEBS Journal. 2007;274:265 - 306. doi:10.1111/ejb.2007.274.issue-s110.1111/j.0014-2956.2007.05861_6.x
Laizé V, Viegas CSB, Price PA, M. Cancela L. Identification of an osteocalcin isoform in fish with a large acidic prodomain. Journal of Biological Chemistry. 2006;281(22):15037-15043. doi:10.1074/jbc.M600373200
Braga D, Laizé V, Tiago DM, M. Cancela L. Enhanced DNA transfer into fish bone cells using polyethylenimine. Molecular Biotechnology. 2006;34(1):51-54. doi:10.1385/MB:34:110.1385/MB:34:1:51
Simões B, Conceição N, Viegas CSB, et al. Identification of a promoter element within the zebrafish colXalpha1 gene responsive to runx2 isoforms Osf2/Cbfa1 and til-1 but not to pebp2alphaA2. Calcif Tissue Int. 2006;79(4):230-44. doi:10.1007/s00223-006-0111-6
Rafael MS, Laizé V, M. Cancela L. Identification of Sparus aurata bone morphogenetic protein 2: molecular cloning, gene expression and in silico analysis of protein conserved features in vertebrates. Bone. 2006;39(6):1373-81. doi:10.1016/j.bone.2006.06.021
Gavaia P, Simes DC, Ortiz-Delgado JB, et al. Osteocalcin and matrix Gla protein in zebrafish (Danio rerio) and Senegal sole (Solea senegalensis): comparative gene and protein expression during larval development through adulthood. Gene Expr Patterns. 2006;6(6):637-52. doi:10.1016/j.modgep.2005.11.010
Ortiz-Delgado JB, Simes DC, Viegas CSB, Schaff BJ, Sarasquete C, M. Cancela L. Cloning of matrix Gla protein in a marine cartilaginous fish, Prionace glauca: preferential protein accumulation in skeletal and vascular systems. Histochem Cell Biol. 2006;126(1):89-101. doi:10.1007/s00418-005-0125-6
Novoa B, Romero A, Mulero V, Rodríguez I, Fernández I, Figueras A. Zebrafish (Danio rerio) as a model for the study of vaccination against viral haemorrhagic septicemia virus (VHSV). Vaccine. 2006;24(31-32):5806 - 5816. doi:10.1016/j.vaccine.2006.05.015
Fonseca VG, Lago-Lestón A, Laizé V, M. Cancela L. Rapid identification of differentially expressed genes by in situ screening of bacteria. Molecular Biotechnology. 2005;30(2):163-166. doi:10.1385/MB:30:210.1385/MB:30:2:163
Laizé V, Martel P, Viegas CSB, Price PA, M. Cancela L. Evolution of matrix and bone  gamma-carboxyglutamic acid proteins in vertebrates. Journal of Biological Chemistry. 2005;280(29):26659-26668. doi:10.1074/jbc.M500257200
Laizé V, Pombinho AR, M. Cancela L. Characterization of Sparus aurata osteonectin cDNA and in silico analysis of protein conserved features: evidence for more than one osteonectin in Salmonidae. Biochimie. 2005;87(5):411-20. doi:10.1016/j.biochi.2005.01.008
Laizé V, Martel P, Viegas CSB, Price PA, M. Cancela L. Evolution of matrix and bone gamma-carboxyglutamic acid proteins in vertebrates. J Biol Chem. 2005;280(29):26659-68. doi:10.1074/jbc.M500257200
Ortiz-Delgado JB, Simes DC, Gavaia P, Sarasquete C, M. Cancela L. Osteocalcin and matrix GLA protein in developing teleost teeth: identification of sites of mRNA and protein accumulation at single cell resolution. Histochem Cell Biol. 2005;124(2):123-30. doi:10.1007/s00418-005-0015-y
Pinto JP, Conceição NM, Viegas CSB, et al. Identification of a new pebp2alphaA2 isoform from zebrafish runx2 capable of inducing osteocalcin gene expression in vitro. J Bone Miner Res. 2005;20(8):1440-53. doi:10.1359/JBMR.050318
Pinto JP, Conceição NM, Viegas CSB, et al. Identification of a New pebp2αA2 Isoform From Zebrafish runx2 Capable of Inducing Osteocalcin Gene Expression In Vitro. Journal of Bone and Mineral Research. 2005;20(8):1440 - 1453. doi:10.1359/JBMR.050318
Engrola S, Conceição LEC, Gavaia P, M. Cancela L, Dinis MTeresa. Effect of pre-weaning feeding regime on weaning performance of Senegalese sole, Solea senegalensis (Kaup, 1858). Israeli Journal of Aquaculture . 2005;57(1).
Elandalloussi LM, Rodrigues P, Afonso R, Leite RB, Nunes PA, M. Cancela L. Shikimate and folate pathways in the protozoan parasite, Perkinsus olseni. Mol Biochem Parasitol. 2005;142(1):106-9. doi:10.1016/j.molbiopara.2005.03.014
Elworthy S, Pinto JP, Pettifer A, M. Cancela L, Kelsh RN. Phox2b function in the enteric nervous system is conserved in zebrafish and is sox10-dependent. Mech Dev. 2005;122(5):659-69. doi:10.1016/j.mod.2004.12.008
Conceição N, Silva AC, Fidalgo J, Belo JA, M. Cancela L. Identification of alternative promoter usage for the matrix Gla protein gene. Evidence for differential expression during early development in Xenopus laevis. FEBS J. 2005;272(6):1501-10. doi:10.1111/j.1742-4658.2005.04590.x
Frazão C, Simes DC, Coelho R, et al. Structural evidence of a fourth Gla residue in fish osteocalcin: biological implications. Biochemistry. 2005;44(4):1234-42. doi:10.1021/bi048336z
Elandalloussi LM, Leite RM, Afonso R, et al. Development of a PCR-ELISA assay for diagnosis of Perkinsus marinus and Perkinsus atlanticus infections in bivalve molluscs. Mol Cell Probes. 2004;18(2):89-96. doi:10.1016/j.mcp.2003.09.008
Simes DC, Williamson MK, Schaff BJ, et al. Characterization of osteocalcin (BGP) and matrix Gla protein (MGP) fish specific antibodies: validation for immunodetection studies in lower vertebrates. Calcif Tissue Int. 2004;74(2):170-80. doi:10.1007/s00223-003-0079-4
Pombinho AR, Laizé V, Molha DM, Marques SMP, M. Cancela L. Development of two bone-derived cell lines from the marine teleost Sparus aurata; evidence for extracellular matrix mineralization and cell-type-specific expression of matrix Gla protein and osteocalcin. Cell Tissue Res. 2004;315(3):393-406. doi:10.1007/s00441-003-0830-1
Streiff R, Mira S, Castro M, M. Cancela L. Multiple paternity in Norway lobster (Nephrops norvegicus L.) assessed with microsatellite markers. Mar Biotechnol (NY). 2004;6(1):60-6. doi:10.1007/s10126-003-0015-7
Gavaia P, Ortiz JB, Simes DC, Sarasquete MC, M. Cancela L. SIMULTANEOUS DETECTION OF OSTEOCALCIN AND MATRIX GLA PROTEIN IN DEVELOPMENTAL STAGES OF ZEBRA FISH (DANIO RERIO). Calcified Tissue International. 2003;72:314 - 468. doi:10.1007/s00223-003-5002-5
Aurelle D, Guillemaud T, Afonso P, et al. Genetic study of Coris julis (Osteichthyes, Perciformes, Labridae) evolutionary history and dispersal abilities. C R Biol. 2003;326(8):771-85.
Elandalloussi LM, Afonso R, Nunes PA, M. Cancela L. Effect of desferrioxamine and 2,2'-bipyridyl on the proliferation of Perkinsus atlanticus. Biomol Eng. 2003;20(4-6):349-54.
Pinto JP, Conceição N, Gavaia P, M. Cancela L. Matrix Gla protein gene expression and protein accumulation colocalize with cartilage distribution during development of the teleost fish Sparus aurata. Bone. 2003;32(3):201-10.
Simes DC, Williamson MK, Ortiz-Delgado JB, Viegas CSB, Price PA, M. Cancela L. Purification of matrix Gla protein from a marine teleost fish, Argyrosomus regius: calcified cartilage and not bone as the primary site of MGP accumulation in fish. J Bone Miner Res. 2003;18(2):244-59. doi:10.1359/jbmr.2003.18.2.244
Robledo JAF, Nunes PA, M. Cancela L, Vasta GR. Development of an in vitro clonal culture and characterization of the rRNA gene cluster of Perkinsus atlanticus, a protistan parasite of the clam Tapes decussatus. J Eukaryot Microbiol. 2002;49(5):414-22.
Viegas CSB, Pinto JP, Conceição N, Simes DC, M. Cancela L. Cloning and characterization of the cDNA and gene encoding Xenopus laevis osteocalcin. Gene. 2002;289(1-2):97-107.
Conceição N, Henriques NM, Ohresser MCP, Hublitz P, Schüle R, M. Cancela L. Molecular cloning of the Matrix Gla Protein gene from Xenopus laevis. Functional analysis of the promoter identifies a calcium sensitive region required for basal activity. Eur J Biochem. 2002;269(7):1947-56.
Gavaia P, Dinis MT, M. Cancela L. Osteological development and abnormalities of the vertebral column and caudal skeleton in larval and juvenile stages of hatchery-reared Senegal sole (Solea senegalensis). Aquaculture. 2002;211(1-4):305 - 323. doi:10.1016/S0044-8486(02)00167-9
Gavaia P, Viegas CSB, Pinto JP, Sarasquete MC, M. Cancela L. DETECTION AND LOCALIZATION OF OSTEOCALCIN (BGP OR BONE GLA PROTEIN) IN TELEOSTS BY IN SITU HYBRIDATION AND IMMUNOHISTOCHEMISTRY. Bone. 2001;28:S101 - S248. doi:10.1016/S8756-3282(01)00454-9
M. Cancela L, Ohresser MC, Reia JP, Viegas CSB, Williamson MK, Price PA. Matrix Gla protein in Xenopus laevis: molecular cloning, tissue distribution, and evolutionary considerations. J Bone Miner Res. 2001;16(9):1611-21. doi:10.1359/jbmr.2001.16.9.1611
Pinto JP, Ohresser MC, M. Cancela L. Cloning of the bone Gla protein gene from the teleost fish Sparus aurata. Evidence for overall conservation in gene organization and bone-specific expression from fish to man. Gene. 2001;270(1-2):77-91.
Carvalho MC, Streiff R, Guillemaud T, Afonso P, Santos RS, M. Cancela L. Isolation and characterization of polymorphic microsatellite markers in Abudefduf luridus (Pisces: Pomacentridae). Mol Ecol. 2000;9(7):993-4.
Guillemaud T, Almada F, M. Cancela L. Interspecific Utility of Microsatellites in Fish: A Case Study of (CT)(n) and (GT)(n) Markers in the Shanny Lipophrys pholis (Pisces: Blenniidae) and Their Use in Other Blennioidei. Mar Biotechnol (NY). 2000;2(3):248-253.
Guillemaud T, Streiff R, R Santos S, Afonso P, Morato T, M. Cancela L. Microsatellite characterization in the rainbow wrasse Coris julis (Pisces: labridae). Mol Ecol. 2000;9(5):631-2.
Gavaia P, Sarasquete C, M. Cancela L. Detection of Mineralized Structures in Early Stages of Development of Marine Teleostei Using a Modified Alcian Blue-Alizarin Red Double Staining Technique for Bone and Cartilage. Biotechnic & Histochemistry. 2000;75(2):79 - 84. doi:10.3109/10520290009064151
Kirfel J, Kelter M, M. Cancela L, Price PA, Schüle R. Identification of a novel negative retinoic acid responsive element in the promoter of the human matrix Gla protein gene. Proc Natl Acad Sci U S A. 1997;94(6):2227-32.
M. Cancela L, Hu B, Price PA. Effect of cell density and growth factors on matrix GLA protein expression by normal rat kidney cells. J Cell Physiol. 1997;171(2):125-34. doi:10.1002/(SICI)1097-4652(199705)171:2<125::AID-JCP2>3.0.CO;2-Q
M. Cancela L, Williamson MK, Price PA. Amino-acid sequence of bone Gla protein from the African clawed toad Xenopus laevis and the fish Sparus aurata. Int J Pept Protein Res. 1995;46(5):419-23.
M. Cancela L, Williamson MK, Price PA. The putative RGD-dependent cell adhesion activity of matrix Gla protein is due to higher molecular weight contaminants. J Biol Chem. 1994;269(16):12185-9.
Rannels SR, M. Cancela L, Wolpert EB, Price PA. Matrix Gla protein mRNA expression in cultured type II pneumocytes. Am J Physiol. 1993;265(3 Pt 1):L270-8.
M. Cancela L, Price PA. Retinoic acid induces matrix Gla protein gene expression in human cells. Endocrinology. 1992;130(1):102-8. doi:10.1210/endo.130.1.1727694
M. Cancela L, Ishida H, Bishop JE, Norman AW. Local chromatin changes accompany the expression of the calbindin-D28K gene: tissue specificity and effect of vitamin D activation. Mol Endocrinol. 1992;6(3):468-75. doi:10.1210/mend.6.3.1584219
M. Cancela L, Hsieh CL, Francke U, Price PA. Molecular structure, chromosome assignment, and promoter organization of the human matrix Gla protein gene. J Biol Chem. 1990;265(25):15040-8.
Rebut-Bonneton C, Ly SY, M. Cancela L. Calcium-transport in quiescent and 1,25-dihydroxycholecalciferol-stimulated human osteosarcoma cells. Role in 24 hydroxylase enhancement. Horm Metab Res. 1988;20(6):339-43. doi:10.1055/s-2007-1010831
de Vernejoul MC, Benamout MP, M. Cancela L, et al. [Primary hyperparathyroidism seen in rheumatology. Clinical symptoms and the relation between bone histologic signs and biological parameters]. Rev Rhum Mal Osteoartic. 1988;55(7):489-94.
Minghetti PP, M. Cancela L, Fujisawa Y, Theofan G, Norman AW. Molecular structure of the chicken vitamin D-induced calbindin-D28K gene reveals eleven exons, six Ca2+-binding domains, and numerous promoter regulatory elements. Mol Endocrinol. 1988;2(4):355-67. doi:10.1210/mend-2-4-355
Parisi M, M. Cancela L. Time course studies on phosphate transfer in frog urinary bladder. Kidney Int. 1988;33(1):58-63.
M. Cancela L, Nemere I, Norman AW. 1 alpha,25(OH)2 vitamin D3: a steroid hormone capable of producing pleiotropic receptor-mediated biological responses by both genomic and nongenomic mechanisms. J Steroid Biochem. 1988;30(1-6):33-9.
M. Cancela L, Rebut-Bonneton C. Regulation of 24-hydroxylase activity in mouse skin fibroblast by cholecalciferol derivatives, triamcinolone acetonide and a calcium modulating agent, nicardipine. J Steroid Biochem. 1987;28(5):479-84.
M. Cancela L, Marie PJ, Le Boulch N, Miravet L. Lack of biological activity of vitamin D3-3 beta sulfate during lactation in vitamin D-deficient rats. Reprod Nutr Dev. 1987;27(6):979-97.
Le Boulch N, M. Cancela L, Miravet L. Calcidiol in human milk. The effect of prohormone on vitamin D status of breast fed unsupplemented infants. Endocrinol Exp. 1986;20(2-3):325-8.
M. Cancela L, Le Boulch N, Miravet L. Relationship between the vitamin D content of maternal milk and the vitamin D status of nursing women and breast-fed infants. J Endocrinol. 1986;110(1):43-50.
Le Boulch N, M. Cancela L, Miravet L, Lange C. Assessment of vitamin D sulphate in human milk using desorption chemical ionization mass spectrometry. Biomed Environ Mass Spectrom. 1986;13(2):53-6.
Marie PJ, M. Cancela L, Le Boulch N, Miravet L. Bone changes due to pregnancy and lactation: influence of vitamin D status. Am J Physiol. 1986;251(4 Pt 1):E400-6.
Gruson M, M. Cancela L, Denne MA, Miravet L. Relationship between bone GLA-protein (BGP) and calcidiol (25-hydroxycalciferol) in serum of breast-fed infants. Endocrinol Exp. 1986;20(2-3):329-34.
M. Cancela L, Marie PJ, Le Boulch N, Miravet L. Influence of vitamin D on mineral metabolism, hormonal status and bone histology in lactating rats and their pups. J Endocrinol. 1985;105(3):303-9.
Rebut-Bonneton C, Demignon J, M. Cancela L, Miravet L. Effect of 25-hydroxyvitamin D3 and 1,25-dihydroxyvitamin D3 maternal loads on maternal and fetal vitamin D metabolite levels in the rat. Reprod Nutr Dev. 1985;25(3):583-90.
M. Cancela L, Marie PJ, Le Boulch N, Miravet L. Vitamin D3 3 beta sulfate has less biological activity than free vitamin D3 during pregnancy in rats. Biol Neonate. 1985;48(5):274-84.
M. Cancela L, Le Boulch N, Miravet L. Action of vitamin D3-3 beta-sulfate during lactation in the rat. World Rev Nutr Diet. 1984;43:153-7.
Boulch NL, M. Cancela L, Miravet L. Cholecalciferol sulfate identification in human milk by HPLC. Steroids. 1982;39(4):391-8.
de Vernejoul MC, Girot R, Gueris J, et al. Calcium phosphate metabolism and bone disease in patients with homozygous thalassemia. J Clin Endocrinol Metab. 1982;54(2):276-81. doi:10.1210/jcem-54-2-276
Projects
Activities
Activity type 
Other Events
This Friday, the Master's student Michele Loiodice, will present and defend his thesis at the University of Algarve at 14:00 pm.
November 8, 2019
Activity type 
Other Events
This Friday, our PhD candidate Patrícia Diogo will defend her thesis about cryopreservation of zebrafish germ cells.
October 18, 2019
at 
Anfiteatro Verde, Edf 8
Activity type 
Scientific Gathering
We thank all participants for joining us at IAFSB, thus making this conference possible. IAFSB conferences would also not be possible without many hours of volunteer work of the local organising...
April 16, 2018 to April 19, 2018
at 
Hotel Vila Galé Albacora, Tavira, Algarve, Portugal
21st April 2017 Organization: CCMAR Location: Universidade do Algarve - Campus de Gambelas (limited places)
April 21, 2017
at 
CCMAR - Universidade do Algarve - Campus de Gambelas (Faro)
Activity type 
Seminar
This wednesday, we will receive Gil Martins (CCMAR) and Aldric Negrier (Reprapalgarve). Don't miss this seminar and the opportunity to get to know about 3D printers and all the potential to use it...
March 8, 2017
at 
Anf. 1.8 (bdg 8) - Gambelas Campus UAlg
Activity type 
Seminar
CCMAR SEMINARS 8th FEB | 13:30 | Anf. 1.8 (Edf. 8) Vitamin K in Fish Skeletal Biology Paulo Gavaia (CCMAR - BIOSKEL) Poster Abstract Vitamin K (VK) is a fat soluble vitamin discovered by Henrik Dam...
February 8, 2017
at 
Anf. 1.8 (edf. 8) - Gambelas Campus UAlg
This course was designed to provide basic knowledge on the use of the biological model zebrafish with main emphasis on its use in the context of the study of human diseases and biomedical sciences...
May 12, 2016
Activity type 
Scientific Gathering
April 27, 2015 to April 30, 2015
at 
Tavira, Algarve, Portugal
Activity type 
Scientific Gathering
April 22, 2013 to April 24, 2013
at 
Tavira, Algarve, Portugal
Activity type 
Scientific Gathering
April 26, 2011 to April 28, 2011
at 
Tavira, Algarve, Portugal
Activity type 
Scientific Gathering
April 27, 2009 to April 29, 2009
at 
Tavira, Algarve, Portugal
News
News 
Com mais esta descoberta, os investigadores acreditam estar cada vez mais perto de novas terapêuticas para doenças ósseas como a osteoporose, que é caraterizada pela perda de massa óssea. O trabalho recentemente publicado pelos...
"O peixe zebra consegue regenerar a sua barbatana caudal, apesar desta ser feita de tecido e osso como os membros dos demais animais e, claro, dos seres humanos. Uma particularidade que foi investigada pelo grupo Bioskel, do...
The network “Strategies of development and improvement of the production of fish larvae in Iberoamerica” (Network CYTED LARVAplus) has organized the kickoff meeting at the Omar Dengo Campus from Universidad Nacional (UNA, Costa...
CCMAR organizes this week the fifth conference “Interdisciplinary Approaches in Fish Skeletal Biology (IAFSB)” in Tavira, Portugal.
Débora Varela is collaborating with the Bioskel Group at CCMAR. Welcome Débora!
Daniela de Castro will join the Bioskel group, where she will prepare her master thesis about microalgae.