Interspecific Utility of Microsatellites in Fish: A Case Study of (CT)(n) and (GT)(n) Markers in the Shanny Lipophrys pholis (Pisces: Blenniidae) and Their Use in Other Blennioidei. | - CCMAR -

Journal Article

TitleInterspecific Utility of Microsatellites in Fish: A Case Study of (CT)(n) and (GT)(n) Markers in the Shanny Lipophrys pholis (Pisces: Blenniidae) and Their Use in Other Blennioidei.
Publication TypeJournal Article
AuthorsGuillemaud, T, Almada, F, M. Cancela, L
Year of Publication2000
JournalMar Biotechnol (NY)
Volume2
Issue3
Date Published2000 May
Pagination248-253
ISSN1436-2236
Abstract

We report the development of new microsatellite markers that can be used for population analyses in the shanny Lipophrys pholis. The procedure involved the construction of a microsatellite-enriched genomic bank. Five (GT)(n) and (CT)(n) microsatellites have been characterized, four of which are polymorphic. The analysis of one population allowed us to verify their usefulness as markers in population studies. Moreover, interspecific amplifications have been performed using primers defined in other species to amplify Lipophrys pholis, or using the primers defined in Lipophrys pholis to amplify other species. We use these results to discuss the hypothesis that microsatellites are highly conserved in fish.

Sapientia

http://www.ncbi.nlm.nih.gov/pubmed/10852803?dopt=Abstract

Alternate JournalMar. Biotechnol.
PubMed ID10852803
CCMAR Authors