Calcium handling in Sparus auratus: effects of water and dietary calcium levels on mineral composition, cortisol and PTHrP levels. | - CCMAR -

Journal Article

TitleCalcium handling in Sparus auratus: effects of water and dietary calcium levels on mineral composition, cortisol and PTHrP levels.
Publication TypeJournal Article
AuthorsAbbink, W, Bevelander, GS, Rotllant, J, Canario, AVM, Flik, G
Year of Publication2004
JournalJ Exp Biol
Volume207
IssuePt 23
Date Published2004 Nov
Pagination4077-84
ISSN0022-0949
KeywordsAnalysis of Variance, Animals, Calcium, Calcium, Dietary, Hydrocortisone, Minerals, Parathyroid Hormone-Related Protein, Phosphorus, Sea Bream, Seawater, Time Factors
Abstract

Juvenile gilthead sea bream (Sparus auratus L.; 10-40 g body mass) were acclimatized in the laboratory to full strength (34 per thousand) or dilute (2.5 per thousand) seawater and fed normal, calcium-sufficient or calcium-deficient diet for nine weeks. Mean growth rate, whole-body calcium and phosphorus content and accumulation rates were determined, as well as plasma levels of ionic and total calcium, cortisol and parathyroid hormone related protein (PTHrP; a hypercalcemic hormone in fish). When confronted with limited calcium access (low salinity and calcium-deficient diet), sea bream show growth arrest. Both plasma cortisol and PTHrP increase when calcium is limited in water or diet, and a positive relationship was found between plasma PTHrP and plasma ionic calcium (R(2)=0.29, N=18, P<0.05). Furthermore, a strong correlation was found between net calcium and phosphorus accumulation (R(2)=0.92, N=16, P<0.01) and between body mass and whole-body calcium (R(2)=0.84, N=25, P<0.01) and phosphorus (R(2)=0.88, N=24, P<0.01) content. Phosphorus accumulation is strongly calcium dependent, as phosphorus accumulation decreases in parallel to calcium accumulation when the diet is calcium deficient but phosphorus sufficient. We conclude that PTHrP and cortisol are involved in the regulation of the hydromineral balance of these fish, with growth-related calcium accumulation as an important target.

DOI10.1242/jeb.01254
Sapientia

http://www.ncbi.nlm.nih.gov/pubmed/15498953?dopt=Abstract

Alternate JournalJ. Exp. Biol.
PubMed ID15498953
CCMAR Authors