|Divergence of duplicate POMC genes in gilthead sea bream Sparus auratus.
|Cardoso, JCR, Laiz-Carrion, R, Louro, B, Silva, N, Canario, AVM, Mancera, JMiguel, Power, DM
|Year of Publication
|Gen Comp Endocrinol
|2011 Sep 15
|Amino Acid Sequence, Animals, Brain, Cloning, Molecular, Fish Proteins, Gene Duplication, Molecular Sequence Data, Phylogeny, Pituitary Gland, Pro-Opiomelanocortin, Sea Bream, Sequence Alignment, Sequence Analysis, Protein
Proopiomelanocorticotrophin (POMC) in vertebrates is produced in the pituitary gland and undergoes post-translational processing to give rise to a range of biologically active peptides. Teleosts possess 2-3 different POMC transcripts which have been proposed to have originated from a whole or partial genome duplication. In the present study 2 transcripts of gilthead sea bream POMC (sbPOMC-α1 and α2) were cloned and characterised. sbPOMC-α1 is expressed principally in the melanotroph cells of the pars intermedia (PI) and sbPOMC-α2 is expressed in the corticotroph cells of the rostral pars distalis and probably also in the PI. The 2 sbPOMC transcripts have a differential tissue distribution in extra-pituitary sites. An appraisal of POMC evolution indicates sbPOMCs belong to one of the two main clades that exist in teleosts and that overall a non conservative process of gene loss occurred in this infraclass.
|Gen. Comp. Endocrinol.