Evaluation of gilthead sea bream, Sparus aurata, sperm quality after cryopreservation in 5 ml macrotubes. | - CCMAR -

Journal Article

TitleEvaluation of gilthead sea bream, Sparus aurata, sperm quality after cryopreservation in 5 ml macrotubes.
Publication TypeJournal Article
AuthorsCabrita, E, Robles, V, Cuñado, S, Wallace, JC, Sarasquete, C, Herráez, MP
Year of Publication2005
Date Published2005 Jun
KeywordsAnimals, Cell Membrane, Cell Survival, Cryopreservation, Fertility, Freezing, Male, Mitochondria, Sea Bream, Semen Preservation, Sperm Motility, Spermatozoa

Cryopreservation produces several types of damage in spermatozoa, leading to fertility impairment. The reduction arises both from a lower viability post-thaw and from sublethal dysfunctions in some of the surviving cells. In the present study, we have analysed the effect of cryopreservation in 5 ml macrotubes on the quality of post-thawed gilthead sea bream sperm. Several standard sperm quality parameters were determined: pH and osmolarity of seminal plasma, sperm concentration, and motility. An exhaustive determination of sperm quality before and after cryopreservation was investigated. Several parameters related with spermatozoal status were determined: ATP content, plasma membrane integrity and functionality, mitochondrial functionality, and sperm fertility. Our results demonstrated that gilthead sea bream spermatozoa suffer several types of damage after freezing/thawing. The percentage of viable cells slightly decreased after cryopreservation, however plasma membrane was affected by cryopreservation, since cells could not resist the hyperosmotic shock. Mitochondrial status was affected by cryopreservation since there was a decrease in the parameters of sperm motility, ATP content (3.17 nmol ATP/10(5) spermatozoa to 1.7 nmol ATP/10(5) spermatozoa in 1:20 frozen samples) and an increase of the percentage of cells with mitochondrial depolarized membranes (11% for fresh and 27% for 1:20 frozen samples). Fertility rate was similar either using fresh or frozen/thawed sperm (77 and 75% hatched larvae, respectively).



Alternate JournalCryobiology
PubMed ID15925579
CCMAR Authors