Regulation of troponin T expression during muscle development in sea bream Sparus auratus Linnaeus: the potential role of thyroid hormones. | - CCMAR -

Journal Article

TitleRegulation of troponin T expression during muscle development in sea bream Sparus auratus Linnaeus: the potential role of thyroid hormones.
Publication TypeJournal Article
AuthorsCampinho, MA, Sweeney, GE, Power, DM
Year of Publication2006
JournalJ Exp Biol
Volume209
IssuePt 23
Date Published2006 Dec
Pagination4751-67
ISSN0022-0949
KeywordsAging, Alternative Splicing, Amino Acid Sequence, Animals, Base Sequence, Gene Expression Regulation, Developmental, Molecular Sequence Data, Muscle, Skeletal, Protein Isoforms, Sea Bream, Thyroxine, Triiodothyronine, Troponin T
Abstract

In the sea bream Sparus auratus three stage-specific fast troponin T (fTnT) isoforms have been cloned and correspond to embryonic-, larval- and adult-specific isoforms. Characterisation, using database searches, of the putative genomic organisation of Fugu rubripes and Tetraodon nigroviridis fTnT indicates that alternative exon splicing in the 5 region of the gene generates the different isoforms. Moreover, comparison of teleost fTnTs suggests that alternative splicing of fTnT appears to be common in teleosts. A different temporal expression pattern for each fTnT splice varotnt is found during sea bream development and probably relates to differing functional demands, as a highly acidic embryonic form (pI 5.16) is substituted by a basic larval form (pI 9.57). Thyroid hormones (THs), which play an important regulatory role in muscle development in flatfish and tetrapods, appear also to influence TnT gene expression in the sea bream. However, THs have a divergent action on different sea bream TnT genes and although the slow isoform (sTnT1) is TH-responsive, fTnT, sTnT2 and the itronless isoform (iTnT) are unaffected. The present results taken together with those published for flatfish seem to suggest differences may exist in the regulation of larval muscle development in teleosts.

DOI10.1242/jeb.02555
Sapientia

http://www.ncbi.nlm.nih.gov/pubmed/17114408?dopt=Abstract

Alternate JournalJ. Exp. Biol.
PubMed ID17114408
CCMAR Authors