Diversity of bacteria in the marine sponge Aplysina fulva in Brazilian coastal waters. | - CCMAR -

Journal Article

TitleDiversity of bacteria in the marine sponge Aplysina fulva in Brazilian coastal waters.
Publication TypeJournal Article
AuthorsHardoim, CCP, Costa, R, Araújo, FV, Hajdu, E, Peixoto, R, Lins, U, Rosado, AS, van Elsas, JD
Year of Publication2009
JournalAppl Environ Microbiol
Volume75
Issue10
Date Published2009 May
Pagination3331-43
ISSN1098-5336
KeywordsAnimals, Bacteria, Biodiversity, Brazil, Cluster Analysis, DNA Fingerprinting, DNA, Bacterial, DNA, Ribosomal, Genes, rRNA, Molecular Sequence Data, Phylogeny, Porifera, RNA, Bacterial, RNA, Ribosomal, 16S, Seawater, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid
Abstract

Microorganisms can account for up to 60% of the fresh weight of marine sponges. Marine sponges have been hypothesized to serve as accumulation spots of particular microbial communities, but it is unknown to what extent these communities are directed by the organism or the site or occur randomly. To address this question, we assessed the composition of specific bacterial communities associated with Aplysina fulva, one of the prevalent sponge species inhabiting Brazilian waters. Specimens of A. fulva and surrounding seawater were collected in triplicate in shallow water at two sites, Caboclo Island and Tartaruga beach, Búzios, Brazil. Total community DNA was extracted from the samples using "direct" and "indirect" approaches. 16S rRNA-based PCR-denaturing gradient gel electrophoresis (PCR-DGGE) analyses of the total bacterial community and of specific bacterial groups--Pseudomonas and Actinobacteria--revealed that the structure of these assemblages in A. fulva differed drastically from that observed in seawater. The DNA extraction methodology and sampling site were determinative for the composition of actinobacterial communities in A. fulva. However, no such effects could be gleaned from total bacterial and Pseudomonas PCR-DGGE profiles. Bacterial 16S rRNA gene clone libraries constructed from directly and indirectly extracted DNA did not differ significantly with respect to diversity and composition. Altogether, the libraries encompassed 15 bacterial phyla and the candidate division TM7. Clone sequences affiliated with the Cyanobacteria, Chloroflexi, Gamma- and Alphaproteobacteria, Actinobacteria, Bacteroidetes, and Acidobacteria were, in this order, most abundant. The bacterial communities associated with the A. fulva specimens were distinct and differed from those described in studies of sponge-associated microbiota performed with other sponge species.

DOI10.1128/AEM.02101-08
Sapientia

http://www.ncbi.nlm.nih.gov/pubmed/19304829?dopt=Abstract

Alternate JournalAppl. Environ. Microbiol.
PubMed ID19304829
PubMed Central IDPMC2681663
CCMAR Authors