Maritime halophyte species from southern Portugal as sources of bioactive molecules. | - CCMAR -

Journal Article

TitleMaritime halophyte species from southern Portugal as sources of bioactive molecules.
Publication TypeJournal Article
AuthorsRodrigues, MJoão, Gangadhar, KN, Vizetto-Duarte, C, Wubshet, SG, Nyberg, NT, Barreira, L, Varela, J, Custódio, L
Year of Publication2014
JournalMar Drugs
Date Published2014 Apr 10
KeywordsAnimals, Anti-Inflammatory Agents, Antineoplastic Agents, Phytogenic, Antioxidants, Biphenyl Compounds, Cell Line, Cell Line, Tumor, Free Radical Scavengers, Humans, Inhibitory Concentration 50, Lipopolysaccharides, Macrophages, Mice, Nitric Oxide, Phenols, Picrates, Plant Extracts, Portugal, Salt-Tolerant Plants

Extracts of five halophytes from southern Portugal (Arthrocnemum macrostachyum, Mesembryanthemum edule, Juncus acutus, Plantago coronopus and Halimione portulacoides), were studied for antioxidant, anti-inflammatory and in vitro antitumor properties. The most active extracts towards the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical were the methanol extracts of M. edule (IC₅₀ = 0.1 mg/mL) and J. acutus (IC₅₀ = 0.4 mg/mL), and the ether extracts of J. acutus (IC₅₀ = 0.2 mg/mL) and A. macrostachyum (IC₅₀ = 0.3 mg/mL). The highest radical scavenging activity (RSA) against the 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical was obtained in the ether extract of J. acutus (IC₅₀ = 0.4 mg/mL) and H. portulacoides (IC₅₀ = 0.9 mg/mL). The maximum total phenolic content (TPC) was found in the methanol extract of M. edule (147 mg gallic acid equivalents (GAE)/g) and in the ether extract of J. acutus (94 mg GAE/g). Significant decreases in nitric oxide (NO) production were observed after incubation of macrophages with lipopolysaccharide (LPS) and the chloroform extract of H. portulacoides (IC₅₀ = 109 µg/mL) and the hexane extract of P. coronopus (IC₅₀ = 98.0 µg/mL). High in vitro cytotoxic activity and selectivity was obtained with the ether extract of J. acutus. Juncunol was identified as the active compound and for the first time was shown to display selective in vitro cytotoxicity towards various human cancer cells.


Alternate JournalMar Drugs
PubMed ID24727393
PubMed Central IDPMC4012463