Regulation of 24-hydroxylase activity in mouse skin fibroblast by cholecalciferol derivatives, triamcinolone acetonide and a calcium modulating agent, nicardipine. | - CCMAR -

Journal Article

TitleRegulation of 24-hydroxylase activity in mouse skin fibroblast by cholecalciferol derivatives, triamcinolone acetonide and a calcium modulating agent, nicardipine.
Publication TypeJournal Article
AuthorsM. Cancela, L, Rebut-Bonneton, C
Year of Publication1987
JournalJ Steroid Biochem
Volume28
Issue5
Date Published1987 Nov
Pagination479-84
ISSN0022-4731
KeywordsAnimals, Calcitriol, Cells, Cultured, Cytochrome P-450 Enzyme System, Dihydroxycholecalciferols, Female, Fibroblasts, Kinetics, Male, Mice, Mice, Inbred Strains, Nicardipine, Skin, Steroid Hydroxylases, Triamcinolone Acetonide, Vitamin D3 24-Hydroxylase
Abstract

Mouse skin fibroblasts in culture were used to study the regulation of 1,25-dihydroxycholecalciferol (1,25(OH)2D3) induced 24 hydroxylase (24-OH-ase) under the influence of 3 agents: (1) 24,25-Dihydroxycholecalciferol (24,25(OH)2D3), 62.5 10(-9) M, which led to a significant decrease in the 1,25(OH)2D3-induced 24-OH-ase, probably acted through a nuclear effect mediated by the 1,25(OH)2D3 receptor protein. (2) Triamcinolone acetonide (10(-6)M) which was found to increase the 24-OH-ase enhancement induced by 1.25 and 6.25 nM 1,25(OH)2D3 whereas it did not alter the effect of 31.2 nM 1,25(OH)2D3. (3) A factor which is likely to induce changes in the cell calcium transport or in the Ca pool sizes, i.e. a calcium channel blocker, nicardipine. The effect of 1.25 nM 1,25(OH)2D3 on 24-OH-ase activity was increased by nicardipine (20 microM) which was found to reduce the effect of 6.25 nM 1,25(OH)2D3. The rate of DNA synthesis (measured by [3H]thymidine incorporation) was increased after incubation of fibroblasts with 1,25(OH)2D3 (1.25 nM) plus triamcinolone acetonide (10(-6) M), although it was reduced by nicardipine in comparison with 1,25(OH)2D3 alone. So the effects of these agents on the 1,25(OH)2D3 induced 24-hydroxylase were shown to be independent of the rate of DNA synthesis.

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http://www.ncbi.nlm.nih.gov/pubmed/3682815?dopt=Abstract

Alternate JournalJ. Steroid Biochem.
PubMed ID3682815
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