Cholecalciferol sulfate identification in human milk by HPLC. | - CCMAR -

Journal Article

TitleCholecalciferol sulfate identification in human milk by HPLC.
Publication TypeJournal Article
AuthorsBoulch, NL, M. Cancela, L, Miravet, L
Year of Publication1982
Date Published1982 Apr
KeywordsCholecalciferol, Chromatography, High Pressure Liquid, Female, Humans, Milk, Human, Pregnancy, Spectrophotometry, Ultraviolet

Synthetic vitamin D3 sulfate was prepared by reacting cholecalciferol with sulfamic acid in pyridine. Vitamin D3 sulfate ammonium salt was purified by crystallisation and transformed in sulfate sodium salt. Homogeneity was controlled by reverse phase high pressure liquid chromatography (HPLC). Purified synthetic vitamin D3 sulfate sodium salt was used as a reference. Milk whey was obtained after protein precipitation by adding ethanol. Vitamin D3 sulfoconjugate was identified in supernatant (lyophylized) after purification by Sephadex LH 20 and HPLC. Milk whey purified fraction obtained exhibited the same ultra-violet absorption (UV) as synthetic vitamin D3 sulfate; after solvolysis, cholecalciferol was liberated from natural and synthetic sulfoconjugate. The results confirmed that vitamin D3 sulfate was present in human milk.


Alternate JournalSteroids
PubMed ID6294930
CCMAR Authors