Chromatography and Mass Spectrometry

This service carries out ultra-high-performance liquid chromatography (UHPLC), preparative chromatography (LC-Prep), high-performance liquid chromatography (HPLC), gas chromatography (GC), and mass spectrometry (UHPLC-MS; GC-MS).

Our services include:
Routine chromatographic analysis for separation, identification and quantification as development, implementation and validation of polar and nonpolar compound.

Ultra Performance Liquid Chromatography (UHPLC) coupled to High Resolution Mass Spectrometry (HRMSn) - Thermo Scientific Ultimate 3000 - Orbitrap Elite

It is a primary tool for compound identification. Provides information on the structure of the main organic and inorganic compounds in each sample, with minimum sample preparation.

The system is a Thermo Scientific instrument, model Nicolet™ iN™10, equipped with DTGS, MCT-A/MCT-B single element and MCT-A Array detectors. Sampling can be performed by ATR, reflection and transmission.

Access: Remote

Technical details

Prices

High-Performance Liquid Chromatograph (HPLC) - (Knauer Smartline + Varian 380 + 2600 UV)

Equipped with fraction collector and UV (Knauer 2600) and evaporative light scattering (ELSD - Varian LC-380) detectors.
This instrument operates in a wavelength range of 190 to 400 nm.

This equipment allows separation, purification, identification, and quantification of organic compounds (liquid samples).

Access: Remote and onsite

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Prices

Gas Chromatographer’s Triple Quadrupole - (Bruker TQ-456GC)

This system can operate in GCMS or GC-FID mode, with PTV and S/SL injectors.
GCMS is an analytical technique that combines the features of gas chromatography and mass spectrometry to identify different substances within a sample.
It allows the identification of organic compounds using NIST libraries and quantification of target compounds by Full scan, SIM and MRM (MS/MS and MSn reduce matrix influences and provide more detailed structural information). Identifies and quantifies volatile organic compounds. This sensitive and simple to operate equipment is used for compound discovery and metabolomics (small molecules/metabolites).
GC-FID is used for separating and analysing compounds that can be vaporized without decomposition. The GC- FID can detect almost all carbon containing compounds.

Access: Remote

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Preparative Chromatography (LC-Prep) - Shimadzu Nexera Prep

The system is equipped with a Light Scattering Detector (ELSD LT II), a UV-Vis Detector (SPD-40), an autosampler and a fraction collector. Four solvents can be used in the mobile phase at pressures up to 400 atm.
The system can be operated under analytical or preparative conditions and therefore the scale up to preparative analysis is straightforward. A Method Scouting Software is available to fast achieve the optimum separation conditions.

Access: Remote

Technical details

Prices under quotation

For more information contact: ccmarcts@ualg.pt