DETECTION AND LOCALIZATION OF OSTEOCALCIN (BGP OR BONE GLA PROTEIN) IN TELEOSTS BY IN SITU HYBRIDATION AND IMMUNOHISTOCHEMISTRY

Paulo Gavaia; Carla SB Viegas; Pinto, JP; Sarasquete, MC; M. Leonor Cancela; Price, P A; Loureiro, Rui M. S.; Pena, Paula C. A.; M. Leonor Cancela; Cavaco, Sofia; Ferreira, Ana; Serra, Manuel; Theuwissen, Elke; Vermeer, Cees; D C Simes

Functional Biochemistry and Proteomics

Short Title

FBP

Descrição

The overall research strategy of FBP is driven primarily by the need to translate strong basic science into clinically relevant advances and promoting transfer of technology, based on the integration of high quality multi-disciplinary academic, researcher and clinical activity with a strong focus on translational research.

Coordenador

Dina Simes

Organiza

Sobre

The overall research strategy of FBP is driven primarily by the need to translate strong basic science into clinically relevant advances and promoting transfer of technology. FBP strategy is based on the integration of high quality multi-disciplinary academic, researcher and clinical activity with a strong focus on translational research.

FBP research team has long expertise in the biochemical aspects of vitamin K-dependent proteins (VKD) and aparticularly unique knowledge about the biochemistry, molecular biology and biomedical aspects of a new VKD member, Gla rich protein (GRP) for which methods and assays are proprietary IP (PCT/PT2009/000046).

Our research interests include the study of GRP and otherkey genes involved in processes of ectopic calcificationand inflammation related diseases such as atherosclerosis, cancer, osteoarthritis, renal disease and diabetes. Through a combination of in vivo studies using human biological samples and in vitro and ex vivo model systems, our research aims to establish new molecular knowledge on the pathophysiology and molecular mechanisms behind the onset and development of these highly prevalentdiseases. The output obtained will most certainly contribute to the prognostic, diagnostic and clinic therapeutics for pathological calcification and inflammation related diseases.

The research activities of FBP also include the exploitation of marine resources and the discovery and validation of natural products derived from marine organisms as novel pharmaceutical, health care and personal products to address key societal challenges. Novel drug leads are tested and developed using pre-clinical assays before clinical testing and translation into medical care or as potential and innovative cosmetics.

Main areas of research

The use of established pre-clinical human cell and tissue culture models, complemented with in vivo studies using a collection of well characterized human samples, for the study ofnovel intervening molecules and molecular mechanisms involved in highly prevalent diseases

Searching for novel disease biomarkers in circulation using collected human biological samples and correlation with clinical information.

Studies on the potential of circulating extracellular vesicles as novel biomarkers and drug delivery systems, with high diagnostic andtherapeutic value for calcification and inflammatory related diseases.
The use of in vitro and ex-vivo cell human modelsas pre-clinical assays for discovery and validationof marine bioactive products as novel diseasemodifying drugs (DMDs) and unveil theirmechanism of action.

Testing bioproducts from marine resources that could become potential and innovative cosmetics.

News

Nuna Araújo ganhou o prémio de melhor oral communication abstract

PRESENÇA LUSA NO CONGRESSO ERA-EDTA

Novo biomarcador ajuda a detetar doenças cardiovasculares em doentes renais

European Perspectives in Cardiology European Perspectives in Cardiology: Cardiovascular research funded by the Portuguese Foundation for Science and Technology

New PhD candidate: Catarina Marreiros

Edição especial: proteínas dependentes de Vitamina K

Membros

Enroll

Ver em tabela

Coordenador	Posição
Dina Simes	Senior Researcher

Nome	Posição
Carla Alexandra São Bento Viegas	Research Assistant
Catarina Marreiros	Research Technician
Dina Simes	Senior Researcher
Hanaa Zbakh	Research Assistant
Nuna Cláudia Peixoto de Araújo	PhD Student

Ver em grelha

Carla Alexandra São Bento Viegas

Posição:

Research Assistant

Catarina Marreiros

Posição:

Research Technician

Dina Simes

Posição:

Senior Researcher

Hanaa Zbakh

Posição:

Research Assistant

Nuna Cláudia Peixoto de Araújo

Posição:

PhD Student

Antigos Membros

Other Past Members

Sofia Cavaco

Marta Rafael

Ruben Costa

Lúcia Santos

Inês Perrolas

Sofia Santos

Inês Luis

Diego Gerini

Kevin Astorga

Galeria

Publicações

Todas publicações


Simes DC, Viegas CSB, Araujo NC. Vitamin K as a Powerful Micronutrient in Aging and Age-Related Diseases: Pros and Cons from Clinical Studies. 2019. Ler mais acerca de Vitamin K as a Powerful Micronutrient in Aging and Age-Related Diseases: Pros and Cons from Clinical Studies Google Scholar
The interplay between mineral metabolism, vascular calcification and inflammation in Chronic Kidney Disease (CKD): challenging old concepts with new facts. 2019. doi:10.18632/aging.102046 Ler mais acerca de The interplay between mineral metabolism, vascular calcification and inflammation in Chronic Kidney Disease (CKD): challenging old concepts with new facts Google Scholar DOI
Viegas C, Edelweiss E, Schneider J, et al. Use of an innovative system and nanotechnology-based strategy for therapeutic applications of Gla-rich protein (GRP). Annals of Medicine. 2019;51(sup1):38 - 38. doi:10.1080/07853890.2018.1561804 Ler mais acerca de Use of an innovative system and nanotechnology-based strategy for therapeutic applications of Gla-rich protein (GRP) Google Scholar DOI
Araújo N, Viegas C, Perrolas I, et al. Amentadione is a new modulating agent for osteoarthritis in an ex-vivo co-culture preclinical assay. Annals of Medicine. 2019;51(sup1):43 - 43. doi:10.1080/07853890.2018.1561895 Ler mais acerca de Amentadione is a new modulating agent for osteoarthritis in an ex-vivo co-culture preclinical assay Google Scholar DOI
Viegas CSB, Simes DC. A dual role for GRP in cardiovascular disease. Aging. 2019;11(5):1323 - 1324. doi:10.18632/aging.v11i510.18632/aging.101851 Ler mais acerca de A dual role for GRP in cardiovascular disease Google Scholar DOI
Viegas CSB, Macedo AL, Matos AA, et al. Translational Research and Innovation in Human and Health Science: Gla-rich protein, a vitamin K-dependent protein involved in inflammation and calcification-related diseases. Annals of Medicine. 2018;50(sup1):S1 - S9. doi:10.1080/07853890.2018.1427452 Ler mais acerca de Translational Research and Innovation in Human and Health Science: Gla-rich protein, a vitamin K-dependent protein involved in inflammation and calcification-related diseases. Google Scholar DOI
Willems BA, Furmanik M, Caron MMJ, et al. Ucma/GRP inhibits phosphate-induced vascular smooth muscle cell calcification via SMAD-dependent BMP signalling. Scientific Reports. 2018;8(1). doi:10.1038/s41598-018-23353-y Ler mais acerca de Ucma/GRP inhibits phosphate-induced vascular smooth muscle cell calcification via SMAD-dependent BMP signalling Google Scholar DOI
Viegas CSB, Santos L, Macedo AL, et al. Chronic Kidney Disease Circulating Calciprotein Particles and Extracellular Vesicles Promote Vascular Calcification. Arteriosclerosis, Thrombosis, and Vascular Biology. 2018;38(3):575 - 587. doi:10.1161/ATVBAHA.117.310578 Ler mais acerca de Chronic Kidney Disease Circulating Calciprotein Particles and Extracellular Vesicles Promote Vascular Calcification Google Scholar DOI
Silva APaula, Viegas C, Simes D, et al. GLA-RICH PROTEIN AS A NOVEL MARKER FOR CALCIFICATIONS IN DIABETIC PATIENTS WITH CKD. Nephrology Dialysis Transplantation. 2018;33(suppl_1):i493 - i493. doi:10.1093/ndt/gfy104.SP430 Ler mais acerca de GLA-RICH PROTEIN AS A NOVEL MARKER FOR CALCIFICATIONS IN DIABETIC PATIENTS WITH CKD Google Scholar DOI
Viegas CSB, Simes DC. Immunity And Inflammation In Health And Disease: Inflammation And Calcification In The Vascular Tree; Insights Into Atherosclerosis. Elsevier; 2018:189 - 201. doi:10.1016/B978-0-12-805417-8.00015-9 Ler mais acerca de Immunity and Inflammation in Health and Disease: Inflammation and Calcification in the Vascular Tree; Insights Into Atherosclerosis Google Scholar DOI
Viegas CSB, Costa RM, Santos L, et al. Gla-rich protein function as an anti-inflammatory agent in monocytes/macrophages: Implications for calcification-related chronic inflammatory diseases. . de Frutos PGarcia, ed. PLOS ONE. 2017;12(5):e0177829. Ler mais acerca de Gla-rich protein function as an anti-inflammatory agent in monocytes/macrophages: Implications for calcification-related chronic inflammatory diseases. Google Scholar DOI
Viegas CSB, Macedo AL, Morais R, et al. Dysregulated fetuin–mineral complexes are linked to vascular calcification in chronic kidney disease: The role of Gla-rich protein. Ultrastructural Pathology. 2017;41(1):78 - 80. doi:10.1080/01913123.2016.1269490 Ler mais acerca de Dysregulated fetuin–mineral complexes are linked to vascular calcification in chronic kidney disease: The role of Gla-rich protein Google Scholar DOI
Morais R, Viegas CSB, Simes DC, Matos APA, Macedo AL. Application of TEM techniques for the study of vascular calcification: Monitoring extracellular vesicles and nanogold immunodetection of fetuin-A, GRP, and CD9. Ultrastructural Pathology. 2017;41(1):130 - 132. doi:10.1080/01913123.2016.1272670 Ler mais acerca de Application of TEM techniques for the study of vascular calcification: Monitoring extracellular vesicles and nanogold immunodetection of fetuin-A, GRP, and CD9 Google Scholar DOI
Viegas C, Santos L, Macedo A, et al. CIRCULATING CALCIPROTEIN PARTICLES AND EXTRACELLULAR VESICLES AS NOVEL PLAYERS IN CHRONIC KIDNEY DISEASE VASCULAR CALCIFICATION. A ROLE FOR GLA-RICH PROTEIN. Nephrology Dialysis Transplantation. 2017;32(suppl_3):iii67 - iii68. doi:10.1093/ndt/gfx123.MO055 Ler mais acerca de CIRCULATING CALCIPROTEIN PARTICLES AND EXTRACELLULAR VESICLES AS NOVEL PLAYERS IN CHRONIC KIDNEY DISEASE VASCULAR CALCIFICATION. A ROLE FOR GLA-RICH PROTEIN Google Scholar DOI
Macedo AL, Viegas CSB, Matos APA, Simes DC. Extracellular Vesicles and Circulating Fetuin-Mineral Complexes in Vascular Calcification; Studies by EM Techniques. Microscopy and Microanalysis. 2016;22(S4):16 - 17. doi:10.1017/S1431927616000271 Ler mais acerca de Extracellular Vesicles and Circulating Fetuin-Mineral Complexes in Vascular Calcification; Studies by EM Techniques Google Scholar DOI
Viegas CSB, Simes DC. New Perspectives for the Nutritional Value of Vitamin K in Human Health. Journal of Nutritional Disorders & Therapy. 2016;6(3). doi:10.4172/2161-050910.4172/2161-0509.1000192 Ler mais acerca de New Perspectives for the Nutritional Value of Vitamin K in Human Health Google Scholar DOI
Viegas CSB, Simes DC. Gla-rich Protein (GRP): A New Player In The Burden Of Vascular Calcification. Journal of Cardiovascular Diseases & Diagnosis. 2016;4(4). doi:10.4172/2329-951710.4172/2329-9517.1000245 Ler mais acerca de Gla-rich Protein (GRP): A New Player In The Burden Of Vascular Calcification Google Scholar DOI
Cavaco S, Viegas CSB, Rafael MS, et al. Gla-rich protein is involved in the cross-talk between calcification and inflammation in osteoarthritis. Cell Mol Life Sci. 2016;73(5):1051-65. doi:10.1007/s00018-015-2033-9 Ler mais acerca de Gla-rich protein is involved in the cross-talk between calcification and inflammation in osteoarthritis. Google Scholar PubMed DOI
Viegas CSB, Rafael MS, Enriquez JL, et al. Gla-rich protein acts as a calcification inhibitor in the human cardiovascular system. Arterioscler Thromb Vasc Biol. 2015;35(2):399-408. doi:10.1161/ATVBAHA.114.304823 Ler mais acerca de Gla-rich protein acts as a calcification inhibitor in the human cardiovascular system. Google Scholar PubMed DOI
Richard N, Engrola S, Palma PS, Simes DC, Conceição LEC. Assessment of protein digestive capacity and metabolic utilisation during ontogeny of Senegalese sole larvae: A tracer study using in vivo produced radiolabelled polypeptide fractions. Aquaculture. 2015;441:35 - 44. doi:10.1016/j.aquaculture.2015.02.003 Ler mais acerca de Assessment of protein digestive capacity and metabolic utilisation during ontogeny of Senegalese sole larvae: A tracer study using in vivo produced radiolabelled polypeptide fractions Google Scholar DOI
Viegas CSB, Rafael MS, Enriquez JL, et al. Gla-rich protein (GRP) is a new player in mineralization-competence of extracellular vesicles involved in vascular calcification. Journal of Vascular Research. 2015;52(1):1 - 88. doi:10.1159/000433498 Ler mais acerca de Gla-rich protein (GRP) is a new player in mineralization-competence of extracellular vesicles involved in vascular calcification Google Scholar DOI
Cavaco S, Williamson MK, Rosa J, et al. Teleost fish osteocalcin 1 and 2 share the ability to bind the calcium mineral phase. Fish Physiol Biochem. 2014;40(3):731-8. doi:10.1007/s10695-013-9880-9 Ler mais acerca de Teleost fish osteocalcin 1 and 2 share the ability to bind the calcium mineral phase. Google Scholar PubMed DOI
Rafael MS, Cavaco S, Viegas CSB, et al. Insights into the association of Gla-rich protein and osteoarthritis, novel splice variants and γ-carboxylation status. Mol Nutr Food Res. 2014;58(8):1636-46. doi:10.1002/mnfr.201300941 Ler mais acerca de Insights into the association of Gla-rich protein and osteoarthritis, novel splice variants and γ-carboxylation status. Google Scholar PubMed DOI
Viegas CSB, Herfs M, Rafael MS, et al. Gla-rich protein is a potential new vitamin K target in cancer: evidences for a direct GRP-mineral interaction. Biomed Res Int. 2014;2014:340216. doi:10.1155/2014/340216 Ler mais acerca de Gla-rich protein is a potential new vitamin K target in cancer: evidences for a direct GRP-mineral interaction. Google Scholar PubMed DOI
Viegas CSB, Simes DC, Williamson MK, et al. Sturgeon osteocalcin shares structural features with matrix Gla protein: evolutionary relationship and functional implications. J Biol Chem. 2013;288(39):27801-11. doi:10.1074/jbc.M113.450213 Ler mais acerca de Sturgeon osteocalcin shares structural features with matrix Gla protein: evolutionary relationship and functional implications. Google Scholar PubMed DOI
Oliveira DV, Silva TS, Cordeiro OD, Cavaco SI, Simes DC. Identification of proteins with potential osteogenic activity present in the water-soluble matrix proteins from Crassostrea gigas nacre using a proteomic approach. ScientificWorldJournal. 2012;2012:765909. doi:10.1100/2012/765909 Ler mais acerca de Identification of proteins with potential osteogenic activity present in the water-soluble matrix proteins from Crassostrea gigas nacre using a proteomic approach. Google Scholar PubMed DOI
Viegas CSB, Conceição N, Fazenda C, Simes DC, M. Cancela L. Expression of Gla-rich protein (GRP) in newly developed cartilage-derived cell cultures from sturgeon ( Acipenser naccarii ). Journal of Applied Ichthyology. 2010;26(2):214 - 218. doi:10.1111/jai.2010.26.issue-210.1111/j.1439-0426.2010.01408.x Ler mais acerca de Expression of Gla-rich protein (GRP) in newly developed cartilage-derived cell cultures from sturgeon ( Acipenser naccarii ) Google Scholar DOI
Roberto VP, Cavaco S, Viegas CSB, et al. Matrix Gla protein in turbot (Scophthalmus maximus): Gene expression analysis and identification of sites of protein accumulation. Aquaculture. 2009;294(3-4):202 - 211. doi:10.1016/j.aquaculture.2009.06.020 Ler mais acerca de Matrix Gla protein in turbot (Scophthalmus maximus): Gene expression analysis and identification of sites of protein accumulation Google Scholar DOI
Viegas CSB, Cavaco S, Neves PL, et al. Gla-rich protein is a novel vitamin K-dependent protein present in serum that accumulates at sites of pathological calcifications. Am J Pathol. 2009;175(6):2288-98. doi:10.2353/ajpath.2009.090474 Ler mais acerca de Gla-rich protein is a novel vitamin K-dependent protein present in serum that accumulates at sites of pathological calcifications. Google Scholar PubMed DOI
Viegas CSB, Simes DC, Laizé V, Williamson MK, Price PA, M. Cancela L. Gla-rich protein (GRP), a new vitamin K-dependent protein identified from sturgeon cartilage and highly conserved in vertebrates. J Biol Chem. 2008;283(52):36655-64. doi:10.1074/jbc.M802761200 Ler mais acerca de Gla-rich protein (GRP), a new vitamin K-dependent protein identified from sturgeon cartilage and highly conserved in vertebrates. Google Scholar PubMed DOI
Rønnestad I, Gavaia P, Viegas CSB, et al. Oligopeptide transporter PepT1 in Atlantic cod (Gadus morhua L.): cloning, tissue expression and comparative aspects. J Exp Biol. 2007;210(Pt 22):3883-96. doi:10.1242/jeb.007898 Ler mais acerca de Oligopeptide transporter PepT1 in Atlantic cod (Gadus morhua L.): cloning, tissue expression and comparative aspects. Google Scholar PubMed DOI
Gavaia P, Simes DC, Ortiz-Delgado JB, et al. Osteocalcin and matrix Gla protein in zebrafish (Danio rerio) and Senegal sole (Solea senegalensis): comparative gene and protein expression during larval development through adulthood. Gene Expr Patterns. 2006;6(6):637-52. doi:10.1016/j.modgep.2005.11.010 Ler mais acerca de Osteocalcin and matrix Gla protein in zebrafish (Danio rerio) and Senegal sole (Solea senegalensis): comparative gene and protein expression during larval development through adulthood. Google Scholar PubMed DOI
Ortiz-Delgado JB, Simes DC, Viegas CSB, Schaff BJ, Sarasquete C, M. Cancela L. Cloning of matrix Gla protein in a marine cartilaginous fish, Prionace glauca: preferential protein accumulation in skeletal and vascular systems. Histochem Cell Biol. 2006;126(1):89-101. doi:10.1007/s00418-005-0125-6 Ler mais acerca de Cloning of matrix Gla protein in a marine cartilaginous fish, Prionace glauca: preferential protein accumulation in skeletal and vascular systems. Google Scholar PubMed DOI
Laizé V, Viegas CSB, Price PA, M. Cancela L. Identification of an osteocalcin isoform in fish with a large acidic prodomain. Journal of Biological Chemistry. 2006;281(22):15037-15043. doi:10.1074/jbc.M600373200 Ler mais acerca de Identification of an osteocalcin isoform in fish with a large acidic prodomain Google Scholar DOI
Simões B, Conceição N, Viegas CSB, et al. Identification of a promoter element within the zebrafish colXalpha1 gene responsive to runx2 isoforms Osf2/Cbfa1 and til-1 but not to pebp2alphaA2. Calcif Tissue Int. 2006;79(4):230-44. doi:10.1007/s00223-006-0111-6 Ler mais acerca de Identification of a promoter element within the zebrafish colXalpha1 gene responsive to runx2 isoforms Osf2/Cbfa1 and til-1 but not to pebp2alphaA2. Google Scholar PubMed DOI
Laizé V, Martel P, Viegas CSB, Price PA, M. Cancela L. Evolution of matrix and bone gamma-carboxyglutamic acid proteins in vertebrates. J Biol Chem. 2005;280(29):26659-68. doi:10.1074/jbc.M500257200 Ler mais acerca de Evolution of matrix and bone gamma-carboxyglutamic acid proteins in vertebrates. Google Scholar PubMed DOI
Ortiz-Delgado JB, Simes DC, Gavaia P, Sarasquete C, M. Cancela L. Osteocalcin and matrix GLA protein in developing teleost teeth: identification of sites of mRNA and protein accumulation at single cell resolution. Histochem Cell Biol. 2005;124(2):123-30. doi:10.1007/s00418-005-0015-y Ler mais acerca de Osteocalcin and matrix GLA protein in developing teleost teeth: identification of sites of mRNA and protein accumulation at single cell resolution. Google Scholar PubMed DOI
Pinto JP, Conceição NM, Viegas CSB, et al. Identification of a new pebp2alphaA2 isoform from zebrafish runx2 capable of inducing osteocalcin gene expression in vitro. J Bone Miner Res. 2005;20(8):1440-53. doi:10.1359/JBMR.050318 Ler mais acerca de Identification of a new pebp2alphaA2 isoform from zebrafish runx2 capable of inducing osteocalcin gene expression in vitro. Google Scholar PubMed DOI
Frazão C, Simes DC, Coelho R, et al. Structural evidence of a fourth Gla residue in fish osteocalcin: biological implications. Biochemistry. 2005;44(4):1234-42. doi:10.1021/bi048336z Ler mais acerca de Structural evidence of a fourth Gla residue in fish osteocalcin: biological implications. Google Scholar PubMed DOI
Pinto JP, Conceição NM, Viegas CSB, et al. Identification of a New pebp2αA2 Isoform From Zebrafish runx2 Capable of Inducing Osteocalcin Gene Expression In Vitro. Journal of Bone and Mineral Research. 2005;20(8):1440 - 1453. doi:10.1359/JBMR.050318 Ler mais acerca de Identification of a New pebp2αA2 Isoform From Zebrafish runx2 Capable of Inducing Osteocalcin Gene Expression In Vitro Google Scholar DOI
Laizé V, Martel P, Viegas CSB, Price PA, M. Cancela L. Evolution of matrix and bone gamma-carboxyglutamic acid proteins in vertebrates. Journal of Biological Chemistry. 2005;280(29):26659-26668. doi:10.1074/jbc.M500257200 Ler mais acerca de Evolution of matrix and bone gamma-carboxyglutamic acid proteins in vertebrates Google Scholar DOI
Simes DC, Williamson MK, Schaff BJ, et al. Characterization of osteocalcin (BGP) and matrix Gla protein (MGP) fish specific antibodies: validation for immunodetection studies in lower vertebrates. Calcif Tissue Int. 2004;74(2):170-80. doi:10.1007/s00223-003-0079-4 Ler mais acerca de Characterization of osteocalcin (BGP) and matrix Gla protein (MGP) fish specific antibodies: validation for immunodetection studies in lower vertebrates. Google Scholar PubMed DOI
Simes DC, Williamson MK, Ortiz-Delgado JB, Viegas CSB, Price PA, M. Cancela L. Purification of matrix Gla protein from a marine teleost fish, Argyrosomus regius: calcified cartilage and not bone as the primary site of MGP accumulation in fish. J Bone Miner Res. 2003;18(2):244-59. doi:10.1359/jbmr.2003.18.2.244 Ler mais acerca de Purification of matrix Gla protein from a marine teleost fish, Argyrosomus regius: calcified cartilage and not bone as the primary site of MGP accumulation in fish. Google Scholar PubMed DOI
Gavaia P, Ortiz JB, Simes DC, Sarasquete MC, M. Cancela L. SIMULTANEOUS DETECTION OF OSTEOCALCIN AND MATRIX GLA PROTEIN IN DEVELOPMENTAL STAGES OF ZEBRA FISH (DANIO RERIO). Calcified Tissue International. 2003;72:314 - 468. doi:10.1007/s00223-003-5002-5 Ler mais acerca de SIMULTANEOUS DETECTION OF OSTEOCALCIN AND MATRIX GLA PROTEIN IN DEVELOPMENTAL STAGES OF ZEBRA FISH (DANIO RERIO) Google Scholar DOI
Viegas CSB, Pinto JP, Conceição N, Simes DC, M. Cancela L. Cloning and characterization of the cDNA and gene encoding Xenopus laevis osteocalcin. Gene. 2002;289(1-2):97-107. Ler mais acerca de Cloning and characterization of the cDNA and gene encoding Xenopus laevis osteocalcin. Google Scholar PubMed
Araújo NCP, Barroca PMM, Bickley JF, et al. Structural effects on sigmatropic shifts in heteroaromatic allyl ethersElectronic supplementary information (ESI) available: selected crystal data for compound 7. See http://www.rsc.org/suppdata/p1/b1/b102674g/. Journal of the Chemical Society, Perkin Transactions 1. 2002;(9):1213 - 1219. doi:10.1039/b102674g Ler mais acerca de Structural effects on sigmatropic shifts in heteroaromatic allyl ethersElectronic supplementary information (ESI) available: selected crystal data for compound 7. See http://www.rsc.org/suppdata/p1/b1/b102674g/ Google Scholar DOI
M. Cancela L, Ohresser MC, Reia JP, Viegas CSB, Williamson MK, Price PA. Matrix Gla protein in Xenopus laevis: molecular cloning, tissue distribution, and evolutionary considerations. J Bone Miner Res. 2001;16(9):1611-21. doi:10.1359/jbmr.2001.16.9.1611 Ler mais acerca de Matrix Gla protein in Xenopus laevis: molecular cloning, tissue distribution, and evolutionary considerations. Google Scholar PubMed DOI
Gavaia P, Viegas CSB, Pinto JP, Sarasquete MC, M. Cancela L. DETECTION AND LOCALIZATION OF OSTEOCALCIN (BGP OR BONE GLA PROTEIN) IN TELEOSTS BY IN SITU HYBRIDATION AND IMMUNOHISTOCHEMISTRY. Bone. 2001;28:S101 - S248. doi:10.1016/S8756-3282(01)00454-9 Ler mais acerca de DETECTION AND LOCALIZATION OF OSTEOCALCIN (BGP OR BONE GLA PROTEIN) IN TELEOSTS BY IN SITU HYBRIDATION AND IMMUNOHISTOCHEMISTRY Google Scholar DOI

Projetos

	Título do Projeto	Coordenador	Período	Financiamento	CCMAR	Total
	Gla-rich protein (GRP) associated to circulating calciprotein particles (CPP) and extracellular vesicles (EVs) as a novel marker of vascular calcification stress in diabetic with chronic kidney disease (CKD)	Carla Alexandra São Bento Viegas, Dina Simes	31/12/2019	Sociedade Portuguesa de Nefrologia	15.00k€
	Characterization of bioengineered extracellular vesicles (EVs) loaded with Gla-rich protein: a proteomic approach	Carla Alexandra São Bento Viegas, Dina Simes		EPIC-XS

	Título do Projeto	Coordenador	Período	Financiamento	CCMAR
PROGLAGRP	Novos conhecimentos sobre o mecanismo de calcificação vascular em doença renal crónica (DRC): o papel da GRP	Carla Alexandra São Bento Viegas	01/05/2013 a 31/12/2015	FCT	150.18k€
BioGlaGRP	Elucidação da função e mecanismo de acção molecular da proteína Gla-rich (GRP) na calcificação vascular (BioGlaGRP)	Carla Alexandra São Bento Viegas	01/02/2012 a 30/09/2015	FCT	159.75k€
OSTEOARTGRP	Nova abordagem para a identificação de alvos terapeuticos da osteoartrite: aplicação da proteomica 2D-DIGE.	Dina Simes	01/01/2011 a 30/06/2014	FCT	150.00k€
BIOMARKGRP	Estudos conducentes ao establecimento da GRP como biomarcador da calcificação vascular	Dina Simes	01/01/2010 a 31/07/2013	FCT	117.74k€
POCI 57921	Função na MGP na regulação local da mineralização da matriz extracelular- Identificação dos motivos estruturais responsáveis por essa função.	Dina Simes	01/09/2005 a 30/06/2008	FCT	86.27k€

Eventos

Tipo de Evento

Seminário

Unveiling the dual role of Gla-rich protein (GRP) in the crosstalk between inflammation and calcification in pathological processes

Carla Viegas will join us on 28 th of June to talk about "Unveiling the dual role of Gla-rich protein (GRP) in the crosstalk between inflammation and calcification in pathological processes". Don't...

Qua, 28/06/2017

Local

Anf. B (CP) - Gambelas Campus UAlg

Notícias

News

Nuna Araújo ganhou o prémio de melhor oral communication abstract

Este abstract tem bastante importância no desenvolvimento de um modelo de teste pré-clínico inovador que irá selecionar os melhores leads para a terapia da Osteoartrose (OA).

PRESENÇA LUSA NO CONGRESSO ERA-EDTA

INVESTIGAÇÃO LUSA A «DAR CARTAS» A 54.ª edição do Congresso da ERA-EDTA (European Renal Association-European Dialysis and Transplant Association), que teve lugar em Madrid, de 3 a 6 de junho 2017, contou com a intervenção de...

Novo biomarcador ajuda a detetar doenças cardiovasculares em doentes renais

Um grupo de investigadores do Centro de Ciências do Mar (CCMAR), em parceria com o Centro Hospitalar Universitário do Algarve e em conjunto com a GenoGla Diagnostics , desenvolveu um novo teste diagnóstico utilizando um...

European Perspectives in Cardiology European Perspectives in Cardiology: Cardiovascular research funded by the Portuguese Foundation for Science and Technology

Carla Alexandra São Bento Viegas, PhD, postdoctoral researcher, Functional Biochemistry and Proteomics group headed by Professor Dina Simes, PhD, Center of Marine Sciences, University of Algarve, Faro

New PhD candidate: Catarina Marreiros

Catarina Marreiro juntou-se ao grupo FBP, onde irá desenvolver o seu PhD sobre a relação das proteínas dependentes de vitamina k com a doença renal crónica e a doença arterial coronária. Bem-vinda!

Edição especial: proteínas dependentes de Vitamina K

As nossas investigadoras estão a procurar colaborações para um Special Issue na área da vitamina K e das Proteínas dependentes da Vitamina K (VKDPs) e os seus impactos na saúde.